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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 7 - 11 (5page)
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초록· 키워드
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It is difficult to introduce DNA in non-invasive manner
into oral cancer cells as well as primary cells for gene
manipulation and expression in vivo. So far, several methods
for a gene delivery have been performed to solve this
problem. Magnetofection is one of the recent methods for
gene transfer, and nanoparticles are applied under a
magnetic field for DNA delivery. We investigated whether
the magnetofection increases the efficiency of a gene
delivery into several oral cell lines. By using a plasmid
coding the green fluorescent protein (GFP), the efficiency of
gene transfer by magnetofection was compared with those
by using the calcium phosphate and the commercial
transfection agent. Indeed, the magnetofection increased
the green fluorescent signal in cells, suggested that this
method apparently enhance the efficiency of gene delivery
without any defects in various oral cancer cell lines. Finally,
we have shown that magnetofection can be a useful
technique for gene delivery to difficult-to-transfect cells to
perform a functional study of genes in vivo.
목차
등록된 정보가 없습니다.
참고문헌 (12)
참고문헌 신청
Edgell CJ / 1998 / Efficient gene transfer to human endothelial cells using DNA complexed to adenovirus particles 25 : 264 ~ 266
Gotschalk S / 1996 / A novel DNA-peptide complex for efficient gene transfer and expression in mammalian cells 3 : 48 ~ 57
Haines AM / 2001 / CL2- A novel cationic peptide for efficient transfection of mammalian cells 8 : 99 ~ 110
Jang Y-J / 2002 / Functional studieson the role of the C-terminal domain of mamalian Polo-like kinase 99 : 1894 ~ 1989
Jang Y-J / 2007 / Inhibition of Polo-like kinase-1 induces apoptosis in oral cancer cels, but not in primary cells
Gotschalk S / 1996 / A novel DNA-peptide complex for efficient gene transfer and expression in mammalian cells 3 : 48 ~ 57
Haines AM / 2001 / CL2- A novel cationic peptide for efficient transfection of mammalian cells 8 : 99 ~ 110
Jang Y-J / 2002 / Functional studieson the role of the C-terminal domain of mamalian Polo-like kinase 99 : 1894 ~ 1989
Jang Y-J / 2007 / Inhibition of Polo-like kinase-1 induces apoptosis in oral cancer cels, but not in primary cells
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