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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 21 - 27 (7page)
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초록· 키워드
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Anginosus group streptococci (AGS) were classified based on the nucleotide sequences of the 16S rRNA gene (16S rDNA) and comprised Streptococcus anginosus, Streptococcus intermedius, and Streptococcus constellatus. It is known that AGS is a causative factor of oral and systematic diseases.
The purpose of this study was to discriminate the 56 clinical strains of AGS isolated from Korean oral cavities using phylogenetic analysis of 16S rDNA and species‐specific PCR at the species-level. The 16S rDNA of clinical strains of AGS was sequenced using the dideoxy chain termination method and analyzed using MEGA version 5 software.
PCR was performed to identify the clinical strains using species‐specific primers described in previous studies and S.
intermedius‐specific PCR primers developed in our laboratory. The resulting phylogenetic data showed that the 16S rDNA sequences can delineate the S. anginosus, S. intermedius,and S. constellatus strains even though the 16S rDNA sequence similarity between S. intermedius and S. constellatus is above 98%. The PCR data showed that each speciesspecific PCR primer pair could discriminate between clinical strains at the species‐level through phylogenetic analysis of 16S rDNA nucleotide sequences. These results suggest that phylogenetic analysis of 16S rDNA and PCR are useful tools for discriminating between AGS strains at the species-level.
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참고문헌 (17)
참고문헌 신청
Allen BL / 2002 / Streptococcus anginosus adheres to vascular endothelium basement membrane and purified extracellular matrix proteins / Microb Pathog 32 : 191 ~ 204
Arirachakaran P / 2012 / Infection of human gingival fibroblasts with Aggregatibacter actinomycetemcomitans: An in vitro study / Arch Oral Biol 57 : 964 ~ 972
Ashimoto A / 1996 / Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions / Oral Micr
Gillis M / 2001 / Polyphasic taxonomy, In Bergey's Manual of Systematic Bacteriology / Springer Verlag : 43 ~ 48
Goto T / 2002 / Rapid identification of Streptococcus intermedius by PCR with the ily gene as a species marker gene / J Med Microbiol 51 : 178 ~ 186
Arirachakaran P / 2012 / Infection of human gingival fibroblasts with Aggregatibacter actinomycetemcomitans: An in vitro study / Arch Oral Biol 57 : 964 ~ 972
Ashimoto A / 1996 / Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions / Oral Micr
Gillis M / 2001 / Polyphasic taxonomy, In Bergey's Manual of Systematic Bacteriology / Springer Verlag : 43 ~ 48
Goto T / 2002 / Rapid identification of Streptococcus intermedius by PCR with the ily gene as a species marker gene / J Med Microbiol 51 : 178 ~ 186
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