지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 13 - 21 (9page)
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초록· 키워드
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Chios gum mastic (CGM) is produced from Pistiacia lentiscus L var chia, which grows only on Chios Island in Greece. CGM is a kind of resin extracted from the stem and leaves, has been used for many centuries in many Mediterranean countries as a dietary supplement and folk medicine for stomach and duodenal ulcers. CGM is known to induce cell cycle arrest and apoptosis in some cancer cells.
This study was undertaken to investigate the alteration of the cell cycle and induction of apoptosis following CGM treatment of HL-60 cells. The viability of the HL-60 cells was assessed using the MTT assay. Hoechst staining and DNA electrophoresis were employed to detect HL-60 cells undergoing apoptosis. Western blotting, immunocytochemistry,confocal microscopy, FACScan flow cytometry, MMP activity and proteasome activity analyses were also employed. CGM treatment of HL-60 cells was found to result in a dose- and time-dependent decrease in cell viability and apoptotic cell death. Tested HL-60 cells showed a variety of apoptotic manifestations and induced the downregulation of G1 cell cycle-related proteins. Taken collectively, our present findings demonstrate that CGM strongly induces G1 cell cycle arrest via the modulation of cell cycle-related proteins,and also apoptosis via proteasome, mitochondrial and caspase cascades in HL-60 cells. Hence, we provide evidence that a natural product, CGM could be considered as a novel therapeutic for human leukemia.
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참고문헌 (26)
참고문헌 신청
Acehan D / 2002 / Three-dimensional structure of the apoptosome: Implications for assembly, procaspase-9 binding, and activation / Mol Cell 9 : 423 ~ 432
Balan KV / 2007 / Antiproliferative activity and induction of apoptosis in human colon cancer cells treated in vitro with constituents of a product derived from Pistacia lent
Barczyk K / 2005 / Serum cytochrome c indicates in vivo apoptosis and can serve as a prognostic marker during cancer therapy / Int J Cancer 21 : 167 ~ 173
Brouckaert, G / 2005 / Apoptotic Pathways and Their Regulation In Apoptotic Pathways as Target for Novel Therapies in Cancer and Other Diseases / Springer Academic Press
Burbano RR / 2009 / Cell cycle arrest induced by pisosterol in HL-60 cells with gene amplification / Cell Biol Toxicol 25 : 245 ~ 251
Balan KV / 2007 / Antiproliferative activity and induction of apoptosis in human colon cancer cells treated in vitro with constituents of a product derived from Pistacia lent
Barczyk K / 2005 / Serum cytochrome c indicates in vivo apoptosis and can serve as a prognostic marker during cancer therapy / Int J Cancer 21 : 167 ~ 173
Brouckaert, G / 2005 / Apoptotic Pathways and Their Regulation In Apoptotic Pathways as Target for Novel Therapies in Cancer and Other Diseases / Springer Academic Press
Burbano RR / 2009 / Cell cycle arrest induced by pisosterol in HL-60 cells with gene amplification / Cell Biol Toxicol 25 : 245 ~ 251
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