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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 109 - 112 (4page)
이용수
초록· 키워드
오류제보하기
Somatic cells nuclear transfer (SCNT) is a useful tool in
studies of developmental biology and animal cloning. However,
SCNT experiments only are allowed to skilled technical
experts. In this experiment, laser-assisted zona pellucida
piercing tool (LASER) was applied in murine SCNT.
LASER minimized the use of piezo-driven micromanipulator
(PIEZO), reducing chances of problems caused
by PIEZO pulses. LASER reduced time that took to pierce
zona pellucida in removal of nucleus from oocyte and
somatic cell injection, which might have taken longer time
with PIEZO. Time and difficulties that took researcher of
equivalent skilled for their experiments were decreased
with LASER, and this might affect the improvement of
embryonic development. (LASER, 6.2% versus PIEZO,
2.9%; P＜ 0.05). Thus, these data support that the use of
LASER can be used for zona pellucida piercing in murine
SCNT program as an alternative to PIEZO.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
A / 1989 / An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro : 679 ~ 688
A / 1991 / Optimization of mouse embryo culture media using simplex methods : 543 ~ 556
Allegra, A / 2007 / Conjoined twins in a trichorionic quadruplet pregnancy after intracytoplasmic sperm injection and quarter laser-assisted zona thinning 87 : 189 ~ 193
Anzai, M / 2006 / Application of laser-assisted zona driling to in vitro fertilization of cryo- preserved mouse oocytes with spermatozoa from a subfertile transgenic mouse 52
Chen, S.-U / 2004 / Technical aspects of the piezo and conventional methods for nuclear transfer of mouse oocytes and their efficiency and efficacy piezo minimizes damage of
A / 1991 / Optimization of mouse embryo culture media using simplex methods : 543 ~ 556
Allegra, A / 2007 / Conjoined twins in a trichorionic quadruplet pregnancy after intracytoplasmic sperm injection and quarter laser-assisted zona thinning 87 : 189 ~ 193
Anzai, M / 2006 / Application of laser-assisted zona driling to in vitro fertilization of cryo- preserved mouse oocytes with spermatozoa from a subfertile transgenic mouse 52
Chen, S.-U / 2004 / Technical aspects of the piezo and conventional methods for nuclear transfer of mouse oocytes and their efficiency and efficacy piezo minimizes damage of
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일상적 치과진료에서 레이저의 사용 A to Z
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LASER에 관한 문헌 고찰
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