지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 289 - 298 (10page)
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초록· 키워드
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Background: The purpose of this study was to investigate perception of hand hygiene and actual hand washing practices of people who used public facilities as well as the presence of indicator bacteria and food-borne pathogens on their hands. Data from this study will be used as a tool for public education and provide basic information on the potential risk for the spread of infectious disease by hands.
Materials and Methods: Sixty S. aureus and 15 methicillin-resistant S. aureus (MRSA)were recovered from 500 swab samples from hands of people in public places, including super markets and amusement facilities in Gwangju Metropolitan City during February to May 2011. Using conventional methods and the Vitek system, all of the isolates were confirmed as Staphylococcus auerus (S. aureus). Antimicrobial susceptibility was determined by performing disk diffusion testing according to the Clinical Laboratory Standard Institute guidelines. The minimum inhibition concentrations (MICs) of MRSA isolates were tested using E-test strips. To confirm the MRSA, polymerase chain reaction (PCR) for the S.aureus-specific gene and mecA gene was performed. Gene detection using PCR, SCCmec typing, Panton-Valentine Leukocidin (PVL), and Multilocus sequence typing (MLST) were performed on all isolates of MRSA.
Results: Of 60 S. aureus isolates, 48 (80%) harbored at least one type of enterotoxin gene: two, three, four, and five types of enterotoxin gene were found in 16 (26.7%),seven (11.7%), 10 (16.7%), and eight (13.3%) isolates, respectively. The most prevalent antimicrobial resistance observed in the S. aureus isolates was to penicillin (92%, 55/60),followed by erythromycin (35%, 21/60), oxacillin (32%, 19/60), and ampicillin (23%,14/60). No resistance was observed against vancomycin, clindamycin, linazolid, rifampin,imipenem, trimethoprim/sulfamethoxazole, and telithromycin. In this study, based on molecular characterization of MRSA isolates, all MRSA, except for one isolate, belonged to ST72 and SCCmec type IV. Eleven of 15 (78.6%) MRSA were ST72:SCCmecIV:t324.
Conclusions: In this study, we detected serious pathogens, including S. aureus and MRSA, from swab samples of peoples’ hands. Most S. aureus isolates harbored the enterotoxin gene and the types of MRSA isolated in this study were communityassociated MRSA, indicating the importance of washing hands for public health.
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참고문헌 (26)
참고문헌 신청
Bean NH. / 1996 / Surveillance for foodborne-disease outbreaks-United States, 1988-1992 / MMWR CDC Surveill Summ 45 : 1 ~ 66
Boschi-Pinto C. / 2008 / Estimating child mortality due to diarrhoea in developing countries / Bull World Health Organ 86 : 710 ~ 717
Burton M. / 2011 / The effect of handwashing with water or soap on bacterial contamination of hands / Int J Environ Res Public Health 8 : 97 ~ 104
Chong Y. / 2000 / Present situation of antimicrobial resistance in Korea / J Infect Chemother 6 : 189 ~ 195
Dinges MM. / 2000 / Exotoxins of Staphylococcus aureus / Clin Microbiol Rev 13 : 16 ~ 34
Boschi-Pinto C. / 2008 / Estimating child mortality due to diarrhoea in developing countries / Bull World Health Organ 86 : 710 ~ 717
Burton M. / 2011 / The effect of handwashing with water or soap on bacterial contamination of hands / Int J Environ Res Public Health 8 : 97 ~ 104
Chong Y. / 2000 / Present situation of antimicrobial resistance in Korea / J Infect Chemother 6 : 189 ~ 195
Dinges MM. / 2000 / Exotoxins of Staphylococcus aureus / Clin Microbiol Rev 13 : 16 ~ 34
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