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자료유형
학술저널
저자정보
저널정보
한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제40권 제3호
발행연도
2013.1
수록면
141 - 146 (6page)

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This study was conducted to examine suspended embryogenic cells growth with days of culture, effects of various kinds/ concentrations of osmoticum for induction of somatic embryos (SEs), following somatic embryos germination or plantlet regeneration. The proliferation pattern of embryogenic cells in suspension culture is characterized by settled cells volume (SCV) increased with the duration of culture with marked the maxium of SCV (10.1 ㎖) in 18 days of culture, however the SCV of cells gradually decreased after that. In comparison of kinds/ concentrations of osmoticum on somatic embryo induction, the highest induction number (352.3/g·FW) of the SE was showed in 0.2 M sucrose, in addition, we also observed some effects with treatments of 0.2 M maltose (203.7) and 0.3 M maltose (193.7), respectively. However, no somatic embryos produced in treatments of 7.5% PEG plus 0.15 M sucrose or maltose. In comparison of germination efficiency of SEs which occurred from the treatments of various kinds/ concentrations of osmoticum, the highest induction frequency of cotyledon (25.2%) was obtained from SEs that produced 0.3 M maltose, however, the best occurrence rates of hypocotyl (39%), radicle (30.3%) and plantlet regeneration (3.5%) were observed from the 0.2 M sucrose treatment, respectively.

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