지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 69 - 74 (6page)
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초록· 키워드
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Using calli of Muscari armeniacum cv. ‘Early Giant’ that is monocotyledonous ornamental bulb crop with increasing demand in Korea, we carried out current studies to establish an in vitro multiple propagation protocol via somatic embryogenesis. We found that soft pale yellow green calli were induced from leaf explants cultured on all media containing 0.1~3.0 mg･L-1 auxins such as 1-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). However, induced calli showed vigorous growth only when they further transferred on same media containing 2,4-D, 4-amino-3,5,6-tri-chloropicolinic acid (picloram), or 3,6-dichloro-o-anisic acid (dicamba). Although frequency of somatic embryo induction depended on callus source and PGR composition in somatic embryo induction media,somatic embryogenesis was initiated on surface of proliferated calli after transferring on media with no PGR or 0.01 mg･L-1 NAA co-supplemented with various cytokinins such as N6-benzylaminopurine (BAP). Highest number of embryo at 9.3 per callus clump was obtained when calli which were grown under 0.1 mg･L-1 picloram supplementation were sub-cultured on medium with 0.01 mg･L-1 NAA and 0.5 mg･L-1 BAP. In addition, morphological characteristics of somatic embryo were categorized into following nine phases: globular, biased heart, biased torpedo, early cotyledonary, middle cotyledonary, late cotyledonary, early sprouting, middle sprouting, and late sprouting embryos.
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참고문헌 (21)
참고문헌 신청
Bae HC / 2000 / Effect of scaling time and scale position on bublet formation in scaling of Muscari armeniacum ‘Early Giant’ / J Kor Soc Hort Sci 41 : 93 ~ 95
Choi ST / 2000 / Effect of leaf cutting time and leaf part on bulblet formation of Muscari armeniacum ‘Early Giant’ / J Kor Soc Hort Sci 41 : 87 ~ 89
Chung JD / 1995 / Somatic embryogenesis from filament-derived callus of Paeonia lactiflora Pall / Korean J Plant Tissue Culture 22 : 47 ~ 51
Dodeman VL / 1997 / Zygotic embryogenesis versus somatic embryogenesis / J Experiment Bot 48 : 1493 ~ 1509
Hartmann HT / 1990 / Plant propagation, principles and practices / Prentice Hall : 205 ~ 206
Choi ST / 2000 / Effect of leaf cutting time and leaf part on bulblet formation of Muscari armeniacum ‘Early Giant’ / J Kor Soc Hort Sci 41 : 87 ~ 89
Chung JD / 1995 / Somatic embryogenesis from filament-derived callus of Paeonia lactiflora Pall / Korean J Plant Tissue Culture 22 : 47 ~ 51
Dodeman VL / 1997 / Zygotic embryogenesis versus somatic embryogenesis / J Experiment Bot 48 : 1493 ~ 1509
Hartmann HT / 1990 / Plant propagation, principles and practices / Prentice Hall : 205 ~ 206
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