지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 27 - 36 (10page)
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초록· 키워드
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We isolated and functionally characterized a Dendrobium Actin1 (DmACT1) promoter that drives strong gene expression in the orchid genus Dendrobium. A genomic fragment containing the region 3227 bp upstream of the coding region of DmACT1 was obtained by inverse PCR.
Detailed comparison of the full-length cDNA and genomic sequences revealed that DmACT1 has a 1374 bp first intron in the 5’ UTR. However, the 5’ flanking sequences upstream of the coding region showed no obvious sequence similarities compared to those of known promoters, including plant actin promoters. Serial deletion constructs of the 5’ flanking region from the translation initiation codon were fused to the coding sequence of a GUS/luciferase fusion reporter to identify the regulatory elements necessary for promoter activity. Transient assays in the flowers of Dendrobium revealed that the 5’ UTR-intron greatly enhanced promoter activity. Moreover, the DmACT1 promoter with its 5’UTR-intron yielded approximately 10-fold higher reporter activity than the rice Act1 promoter-intron. Our data suggest that the DmACT1 promoter with its 5’ UTR-intron is a useful tool for strong expression of transgenes in Dendrobium orchids.
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참고문헌 (25)
참고문헌 신청
An YQ / 1996 / Meagher RB (1996) Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues / Plant J 10 : 107 ~ 121
Bourdon V / 2001 / Introns and their positions affect the translational activity of mRNA in plant cells / EMBO Rep 2 : 394 ~ 398
Bradnam KR / 2008 / Longer first introns are a general property of eukaryotic gene structure / PloS one 3 : e3093 ~
Castillo-Davis CI / 2002 / Selection for short introns in highly expressed genes / Nat Genet 31 : 415 ~ 418
Chia TF / 1994 / Chua NH(1994)The firefly luciferase gene as a non-invasive reporter for Dendrobium transformation / Plant J 6 : 441 ~ 446
Bourdon V / 2001 / Introns and their positions affect the translational activity of mRNA in plant cells / EMBO Rep 2 : 394 ~ 398
Bradnam KR / 2008 / Longer first introns are a general property of eukaryotic gene structure / PloS one 3 : e3093 ~
Castillo-Davis CI / 2002 / Selection for short introns in highly expressed genes / Nat Genet 31 : 415 ~ 418
Chia TF / 1994 / Chua NH(1994)The firefly luciferase gene as a non-invasive reporter for Dendrobium transformation / Plant J 6 : 441 ~ 446
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