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자료유형
학술저널
저자정보
저널정보
한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제35권 제3호
발행연도
2008.1
수록면
229 - 234 (6page)

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An efficient micropropagation was established by using axillary bud explants from two-year-old tree (Echinosphorea koreensis Nakai), which has been known as a rare and endangered species. Among various basal media tested, DKW medium was shown to be the best for axillary shoot elongation. The addition of both BA and TDZ to the medium induced 6 to 10 shoots per explant during eight weeks of culture, without showing any abnormal morphology at the shoot proliferation stage. However, high concentration of TDZ (> 0.05 mg/L) appeared to cause hyperhydration on either leaf or shoot at the later developmental stage. Approximately 20 % of shoots produced roots by the addition of 1.0 mg/L NAA but not by IBA (0.2 ~ 1.0 mg/L). Ex vitro micro-cuttings were better source for root induction; up to 58.6% of the micro-cuttings rooted when 100 mg/L IBA was applied to the soil (vermiculite). More than 90% of plantlets with roots were successfully acclimatized and grew normally in the field. Therefore, we suggest that this endangered tree species can be effectively micropropagated by axillary bud culture system developed in this study.

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