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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 327 - 336 (10page)
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Cysteine proteases have been known as a critical factor in plant defense mechanisms in pineapple, papaya, or wild fig. Papain or ficin is one kind of cysteine proteases that shows toxic effects to herbivorous insects and pathogenic bacteria. However, resistance to bacterial soft rot of plants genetically engineered with cysteine protease has been little examined thus far. We cloned a cysteine protease cDNA from Ananas comosus and ntroduced the gene into Chinese cabbage (Brassica rapa) under the control of the cauliflower mosaic virus 35S promoter. The transgene was stably integrated and actively transcribed in transgenic plants. In comparisons with wild-type plants, the T2 and T3 transgenic plants exhibited a significant increase in endo-protease activity in leaves and enhanced resistance to bacterial soft rot. A cDNA microarray analysis revealed that several genes were more abundantly transcribed in the transgenic than in the wild type. These genes encode a glyoxal oxidase, PR-1 protein, PDF1, protein kinase, LTP protein, UBA protein and protease inhibitor. These results suggest an important role for cysteine protease as a signaling regulator in biotic stress signaling pathways, leading to the build-up of defense mechanism to pathogenic bacteria in plants.
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참고문헌 (23)
참고문헌 신청
Alonso JM / 1995 / A putative vacuolar processing protease is regulated by ethylene and also during fruit ripening in Citrus fruit / Plant Physiol. 109 : 541 ~ 547
Andres M / 2001 / Transgenic plants producing the bacterial pheromone N-acylhomoserine lactone exhibit enhanced resistance to the bacterial phytopathogen Erwinia carotovora /
Barrett AJ / 1986 / Proteinase inhibitors / Amsterdam Science Publishers : 515 ~ 569
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding / Anal. Biochem. 72 :
Buchanan-Wollaston V / 1997 / Leaf senescence in Brassica napus, cloning of senescence related genes by subtractive hybridization / Plant Mol. Biol. 33 : 821 ~ 843
Andres M / 2001 / Transgenic plants producing the bacterial pheromone N-acylhomoserine lactone exhibit enhanced resistance to the bacterial phytopathogen Erwinia carotovora /
Barrett AJ / 1986 / Proteinase inhibitors / Amsterdam Science Publishers : 515 ~ 569
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding / Anal. Biochem. 72 :
Buchanan-Wollaston V / 1997 / Leaf senescence in Brassica napus, cloning of senescence related genes by subtractive hybridization / Plant Mol. Biol. 33 : 821 ~ 843
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