지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 217 - 223 (7page)
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Pueraria lobata, a medicinally important leguminous plant produces various isoflavones including puerarin, daidzin and daidzein which are metabolized to equol via dihydrodaidzein and tetrahydrodaidzein by the bacterial fermentation of natural isoflavone sources in human intestines. In this study, we described callus proliferation and isoflavone production in callus of Korean wild arrowroot and dihydrodaidzein biosynthesis in callus extract fermented with Pediococcus pentosaceus. Proliferation was the best at callus cultured in the medium containing 1.0 mg/L TDZ and 1.0 mg/L NAA at light condition for 12 days. Puerarin was significantly more produced at callus cultured in the medium containing 2.0 mg/L kinetin and 1.0 mg/L NAA at dark condition for 16 days, but daidzin and daidzein were not significant. Callus extract was successfully fermented with P. pentosaceus and dihydrodaidzein, which is one of equol precursors formed by biotransformation, was confirmed to be produced. These results will facilitate mass production of callus and isoflavones as equol precursors from Korean wild arrowroot and can be applied for the production of equol by biotransformation in vitro.
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참고문헌 (23)
참고문헌 신청
Arai Y / 2000 / Comparison of isoflavones among dietary intake, plasma concentration and urinary excretion for accurate estimation of phytoestrogen intake / J Epidemiol 10 :
Barnes S / 1998 / Evolution of the health benefits of soy isoflavones / Proc Soc Exp Biol Med 217 : 386 ~ 392
Boonsnongcheep P / 2010 / Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures / Plant Cell Tiss
Cassidy A / 1996 / Physiological effects of phyto-oestrogens in relation to cancer and other human health risks / Proc Nutr Soc 55 : 399 ~ 417
Chansakaow S / 2000 / Isoflavonoids from Pueraria mirifica and their estrogenic activity / Planta Med 66 : 572 ~ 574
Barnes S / 1998 / Evolution of the health benefits of soy isoflavones / Proc Soc Exp Biol Med 217 : 386 ~ 392
Boonsnongcheep P / 2010 / Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures / Plant Cell Tiss
Cassidy A / 1996 / Physiological effects of phyto-oestrogens in relation to cancer and other human health risks / Proc Nutr Soc 55 : 399 ~ 417
Chansakaow S / 2000 / Isoflavonoids from Pueraria mirifica and their estrogenic activity / Planta Med 66 : 572 ~ 574
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