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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 144 - 148 (5page)
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초록· 키워드
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The transfer of Mn-Superoxide Dismutase (SOD2) gene, complex gene (SA) of CuZnSOD and ascorbate peroxidase (APX), and NDP kinase 2 (NDPK2) gene into Korean 4 cultivars (cvs. Millenium White, Glory Blue, Glory Red, and Glory Purple) and 15 purebred lines of petunia was conducted using Agrobaterium-mediated technique. Two (Wongyo A2-16 and A2-36) of 15 purebred lines and one (cv. Glory Red) of 4 cultivars were effective for the transfer of SOD2 gene. The putative transgenic plants survived on the 2nd selection medium were 124. From PCR analysis, 118 (derived from 4 cultivars and 2 purebred lines) of 124 plants were confirmed to contain marker (npt II) gene, while 58 of 118 plants did not have target genes. There were no plants with both npt II and SA genes. Twenty seven of 28 SOD2 transgenic plants were re-confirmed as transformants by Sothern analysis. SOD2 and NDPK2 genes were expressed in the transgenic petunias as the ratio of 77.8 to 100.0 % and 23.5%, respectively. T1 seeds were obtained from 36 acclimated transgenic plants (SOD2 34 plus NDPK2) in a glasshouse by self-pollination.
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등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
Chandler SF / 2005 / Biotechnology in ornamental horticulture / In Vitro Cell. Biol._Plant 41 : 591 ~ 601
Fang GC / 2002 / The SOD2 gene, encoding a manganese-type superoxide dismutase, is upregulated during conidiogenesis in the plant pathogenic fungus Colletotrichum graminicola
Haejeong M / 2003 / NDP kinase 2 interacts with two oxidative stress-activated MARKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic pla
Kim KY / 2003 / A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cell
Lee SH / 2007 / Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide
Fang GC / 2002 / The SOD2 gene, encoding a manganese-type superoxide dismutase, is upregulated during conidiogenesis in the plant pathogenic fungus Colletotrichum graminicola
Haejeong M / 2003 / NDP kinase 2 interacts with two oxidative stress-activated MARKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic pla
Kim KY / 2003 / A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cell
Lee SH / 2007 / Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide
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