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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 329 - 335 (7page)
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초록· 키워드
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Tocopherols are essential lipophilic antioxidant in human cells, while little is known about its function in plant tissues. To study the impact of composition and content of tocopherols on stress tolerance, tobacco (Nicotiana tabacum) was transformed with a construct containing a cDNA insert encoding γ-tocopherol methyltransferase (γ-TMT/VTE4) from perilla under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic tobacco was confirmed by PCR and RT-PCR. The total content and composition of tocopherols in the transgenic lines were similar with wild type controls. However, chlorophyll-a and carotenoid content in the transgenic lines were increased by up to 45% (P< 0.01) and 39% (P< 0.02), respectively. Also, the over-expression of γ-TMT increased the salt stress tolerance in tobacco plants. These results demonstrate that over-expression of γ-TMT gene in tocopherol bio-synthetic pathway can increase salt stress tolerance and contents of chlorophyll-a and carotenoid in transgenic tobacco plants.
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참고문헌 (22)
참고문헌 신청
Abbasi A. R. / 2007 / Specific roles of α- and γ-tocopherol in abiotic stress responses of transgenic tobacco / Plant Physiol 143 : 1720 ~ 1738
Alscher R. G. E. N. / 2002 / Role of superoxide dismutases (SODs) in controlling oxidative stress in plants / J Exp Bot 53 : 1331 ~ 1341
Bergmuller E. / 2003 / Characterization of an Arabidopsis mutant deficient in gammatocopherol methyltransferase / Plant Mol Biol 52 : 1181 ~ 1190
Dat J. V. S. / 2000 / Dual action of the active oxygen species during plant stress responses / Cell Mol Life Sci 57 : 779 ~ 795
DellaPenna D. / 2006 / Progress in the dissection and manipulation of plant vitamin E biosynthesis. Physiol / Plantarum 126 : 356 ~ 368
Alscher R. G. E. N. / 2002 / Role of superoxide dismutases (SODs) in controlling oxidative stress in plants / J Exp Bot 53 : 1331 ~ 1341
Bergmuller E. / 2003 / Characterization of an Arabidopsis mutant deficient in gammatocopherol methyltransferase / Plant Mol Biol 52 : 1181 ~ 1190
Dat J. V. S. / 2000 / Dual action of the active oxygen species during plant stress responses / Cell Mol Life Sci 57 : 779 ~ 795
DellaPenna D. / 2006 / Progress in the dissection and manipulation of plant vitamin E biosynthesis. Physiol / Plantarum 126 : 356 ~ 368
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