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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 110 - 116 (7page)
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초록· 키워드
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This study was conducted to develop a simple, rapid, and reliable method for in vitro propagation of disease-free and true-to-type clones from sucker explants of thornless blackberry (Rubus fruticosus L. × R. parvifolius L.). To induce multiple shoots, the sucker explants were sterilized in 1% NaOCl solution, and then were aseptically cultured on the full and 1/2 MS solid medium supplemented with BAP (0.1, 0.5, 1.0, 2.0 mg/L). After six weeks of culture, the highest frequency (85.4%) of shoot formation from sucker explants was obtained on the full-strength MS medium with 1.0 mg/L BAP. Node explants obtained from multiple shoots were cultured on the various media of full- or half-strength of AD, B5, MS, SH, QL, WPM media, respectively. After 30 days of culture, plant growth was good on the half-AD, half-QL medium. After 90 days of culture, plant growth was good on the full MS and full SH medium. The survival rate of the plantlets after transfer to plastic pots containing soil mixture (sand: soil: vermiculite was 1:1:1, vol.) in the greenhouse was 98%. The results indicate that a multiple-shoot procedure can be applied for an efficient mass propagation of Rubus fruticosus L. × R. parvifolius L.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Anderson / 1980 / Tissue culture propagation of red and black raspberries (Rubus idaeus and R. occidentalis) / Acta Hort. 112:13~20
Bobrowski VL / 1996 / Micropropagation of blackberries (Rubus sp.) cultivars / Rev. Bras. de Agrociencia 2:17~20
Broome OC / 1978 / In vitro propagation of blackberry / HortSci 13:151~153
Casto BC / 2002 / Chemoprevention of oral cancer by black rasberries / Anticancer Res. 22:4005~4015
Cho MJ / 2004 / Flavonoid glycosides and antioxidant capacity of various blackberry, blueberry and red grape genotypes determined by high-performance liquid chromatography/mass spectrometry / J SCI Food AGR 84(13):771~1782
Bobrowski VL / 1996 / Micropropagation of blackberries (Rubus sp.) cultivars / Rev. Bras. de Agrociencia 2:17~20
Broome OC / 1978 / In vitro propagation of blackberry / HortSci 13:151~153
Casto BC / 2002 / Chemoprevention of oral cancer by black rasberries / Anticancer Res. 22:4005~4015
Cho MJ / 2004 / Flavonoid glycosides and antioxidant capacity of various blackberry, blueberry and red grape genotypes determined by high-performance liquid chromatography/mass spectrometry / J SCI Food AGR 84(13):771~1782
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