지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 272 - 277 (6page)
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초록· 키워드
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Plants expressing Agrobacterium sp. strain CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) are known to be resistant to glyphosate, a potent herbicide that inhibits the activity of the endogenous plant EPSPS. In order to develop herbicide-resistant rice, we prepared transgenic rice plants with CP4 EPSPS gene under the control of CaMV 35S promoter for over-expression. A recombinant plasmid was transformed into rice via Agrobacterium-mediated transformation. A large number of transgenic rice plants were obtained with glyphosate and most of the transformants showed fertile. The integration and expression of CP4 EPSPS gene from regenerated plants was analyzed by Southern and northern blot analysis. The transgenic rice plants had CP4 EPSPS enzyme activity levels more than 15-fold higher than the wild-type plants. EPSPS enzyme activity of transgenic rice plants was also identified by strip-test method. Field trial of transgenic rice plants further confirmed that they can be selectively survived at 100% by spay of glyphosate (Roundup®) at a regular dose used for conventional rice weed control.
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등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
An G / 1988 / Binary vectors, In Plant Molecular Biology Mannual A3 / Kluwer Academic Press, Dordrecht : 1 ~ 19
Baerson SR / 2002 / Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase / Plant Physiol 129 : 1265
Barry G / 1992 / Inhibitors of amino acid biosynthesis: strategies for imparting glyphosate tolerance to crop plants / Curr Top Plant Physiol 7 : 139 ~ 145
Cao J / 1992 / Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells / Plant Cell Rep 11 :
Chu CC / 1975 / Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources / Sci Sin 18 : 659 ~ 668
Baerson SR / 2002 / Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase / Plant Physiol 129 : 1265
Barry G / 1992 / Inhibitors of amino acid biosynthesis: strategies for imparting glyphosate tolerance to crop plants / Curr Top Plant Physiol 7 : 139 ~ 145
Cao J / 1992 / Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells / Plant Cell Rep 11 :
Chu CC / 1975 / Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources / Sci Sin 18 : 659 ~ 668
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