지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 42 - 48 (7page)
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초록· 키워드
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Chloroplast genetic engineering of higher plants offers several unique advantages compared with nuclear genome transformation, such as high levels of transgene expression, a lack of position effect due to site-specific transgene integration by homologous recombination, multigene engineering in a single transformation event and reducing risks of gene flow via pollen due to maternal inheritance. We established a reproducible chloroplast ransformation system of potato using a tobacco specific plastid transformation vector, pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA). Stable transgene integration into chloroplast genomes and the homoplasmic state of the transgenome were confirmed by PCR and Southern blot analyses. Northern, immunoblot analysis, and GFP fluorescence imaging revealed high expression and accumulation of GFP in the plastids of potato leaves. This system would provide new opportunities for genetic improvement and mass production of value added foreign proteins in this crop.
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참고문헌 (38)
참고문헌 신청
Okumura S / 2006 / Transformation of poplar(Populus alba)plastids and expression of foreign proteins in tree chloroplasts / Transgenic Res 15:637~646
Ruf S / 2001 / Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit / Nat Biotechnol 19:870~875
Ruhlman T / 2007 / Expression of cholera toxin B-proinsulin fusion protein in lettuce and tobacco chloroplast-oral administration protects against development of insulitis in non-obese diabetic mice / Plant Biotechnol J 5:495~510
Sikdar SR / 1998 / Plastid transformation in Arabidopsis thaliana / Plant Cell Rep 18:20~24
Singh AK / 2010 / Plastid transformation in eggplant (Solanum melongena L.) / Transgenic Res 19:113~119
Ruf S / 2001 / Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit / Nat Biotechnol 19:870~875
Ruhlman T / 2007 / Expression of cholera toxin B-proinsulin fusion protein in lettuce and tobacco chloroplast-oral administration protects against development of insulitis in non-obese diabetic mice / Plant Biotechnol J 5:495~510
Sikdar SR / 1998 / Plastid transformation in Arabidopsis thaliana / Plant Cell Rep 18:20~24
Singh AK / 2010 / Plastid transformation in eggplant (Solanum melongena L.) / Transgenic Res 19:113~119
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