지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 141 - 149 (9page)
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초록· 키워드
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We have previously isolated dieckol, a nutrient polyphe-nol compound, from the brown alga, Ecklonia cava (Lee et al., 2010a). Dieckol shows both antitumor and antioxidant activity and thus is of special interest for the development of chemopreventive and chemotherapeutic agents against cancer. However, the mechanism by which dieckol exerts its antitumor activity is poorly understood. Here, we show that dieckol, derived from E. cava, inhibits migration and invasion of HT1080 cells by scavenging intracellular reactive oxygen species (ROS). H2O2 or integrin signal-media-ted ROS generation increases migration and invasion of HT1080 cells, which correlates with Rac1 activation and increased expression and phosphorylation of focal adhesion kinase (FAK). Rac1 activation is required for ROS generation. Depletion of FAK by siRNA suppresses Rac1-ROS-induced cell migration and invasion. Dieckol treatment attenuated intracellular ROS levels and activation of Rac1 as well as expression and phosphorylation of FAK. Dieckol treatment also decreases complex formation of FAK-Src-p130Cas and expression of MMP2, 9, and 13. These results suggest that the Rac1-ROS-linked cascade enhances migration and invasion of HT1080 cells by in-ducing expression of MMPs through activation of the FAK signaling pathway, whereas dieckol downregulates FAK signaling through scavenging intracellular ROS. This finding provides new insights into the mechanisms by which dieckol is able to suppress human cancer progresssion and metastasis. Therefore, we suggest that dieckol is a potential therapeutic agent for cancer treatment.
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참고문헌 (21)
참고문헌 신청
Aslan, M. / 2003 / Oxidants in receptor tyrosine kinase signal transduction pathways / Antioxid Redox Signal 5 : 781 ~ 788
Cance, W. G. / 2000 / Immunohistochemical analyses of focal adhesion kinase expression in benign and malignant human breast and colon tissues: correlation with preinvasive an
Cary, L. A. / 1996 / Stimulation of cell migration by overexpression of focal adhesion kinase and its association with Src and Fyn / J. Cell Sci 109 (Pt7) : 1787 ~ 1794
Chan, W. H. / 2003 / Curcumin inhibits UV irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells / J. Cell Biochem 90
Chang, F. / 2007 / FAK potentiates Rac1 activation and localization to matrix adhesion sites: a role for betaPIX / Mol. Biol. Cell 18 : 253 ~ 264
Cance, W. G. / 2000 / Immunohistochemical analyses of focal adhesion kinase expression in benign and malignant human breast and colon tissues: correlation with preinvasive an
Cary, L. A. / 1996 / Stimulation of cell migration by overexpression of focal adhesion kinase and its association with Src and Fyn / J. Cell Sci 109 (Pt7) : 1787 ~ 1794
Chan, W. H. / 2003 / Curcumin inhibits UV irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells / J. Cell Biochem 90
Chang, F. / 2007 / FAK potentiates Rac1 activation and localization to matrix adhesion sites: a role for betaPIX / Mol. Biol. Cell 18 : 253 ~ 264
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