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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 211 - 216 (6page)
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Cnu is a small 71-amino acid protein that complexes with H-NS and binds to a specific sequence in the replication origin of the E. coli chromosome. To understand the me-chanism of interaction between Cnu and H-NS, we used bacterial genetics to select and analyze Cnu variants that cannot complex with H-NS. Out of 2,000 colonies, 40 Cnu variants were identified. Most variants (82.5%) had a single mutation, but a few variants (17.5%) had double amino acid changes. An in vitro assay was used to identify Cnu variants that were truly defective in H-NS binding. The changes in these defective variants occurred exclusively at charged amino acids (Asp, Glu, or Lys) on the surface of the protein. We propose that the attractive force that governs the Cnu-H-NS interaction is an ionic bond, unlike the hydrophobic interaction that is the major attractive force in most proteins.
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참고문헌 (24)
참고문헌 신청
Badaut, C. / 2002 / The degree of oligomerization of the H-NS nucleoid structuring protein is related to specific binding to DNA / J. Biol. Chem 277 : 41657 ~ 41666
Bae, S. H. / 200802 / Structure of the nucleoid-associated protein Cnu reveals common binding sites for H-NS in Cnu and Hha / BIOCHEMISTRY 47 (7) : 1993 ~ 2001
Bloch, V. / 2003 / The H-NS dimerization domain defines a new fold contributing to DNA recognition / Nat. Struct. Biol 10 : 212 ~ 218
Bouffartigues, E. / 2007 / H-NS cooperative binding to high-affinity sites in a regulatory element results in transcriptional silencing / Nat. Struct. Mol. Biol 14 : 441 ~ 44
Cordeiro, T. N. / 2008 / A single residue mutation in Hha preserving structure and binding to H-NS results in loss of H-NS mediated gene repression properties / FEBS Lett 582
Bae, S. H. / 200802 / Structure of the nucleoid-associated protein Cnu reveals common binding sites for H-NS in Cnu and Hha / BIOCHEMISTRY 47 (7) : 1993 ~ 2001
Bloch, V. / 2003 / The H-NS dimerization domain defines a new fold contributing to DNA recognition / Nat. Struct. Biol 10 : 212 ~ 218
Bouffartigues, E. / 2007 / H-NS cooperative binding to high-affinity sites in a regulatory element results in transcriptional silencing / Nat. Struct. Mol. Biol 14 : 441 ~ 44
Cordeiro, T. N. / 2008 / A single residue mutation in Hha preserving structure and binding to H-NS results in loss of H-NS mediated gene repression properties / FEBS Lett 582
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