지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 53 - 58 (6page)
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초록· 키워드
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We constructed deletion mutants and seven point mutants by polymerase chain reaction to investigate the specificity of feline foamy virus integrase functional domains. Complementation reactions were performed for three enzymatic activities such as 3’-end processing, strand transfer, and disintegration. The complementation reactions with deletion mutants showed several activities for 3’-end processing and strand transfer. The conserved central domain and the combination of the N-terminal or C-terminal domains increased disintegration activity significantly. In the complementation reactions between deletion and point mutants, the combination between D107V and deletion mutants revealed 3’-end processing activities, but the combination with others did not have any activity, including strand transfer activities. Disintegration activity increased evenly,except the combination with glutamic acid 200. These results suggest that an intact central domain mediates enzymatic activities but fails to show these activities in the absence of the N-terminal or C-terminal domains.
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참고문헌 (24)
참고문헌 신청
Achong, B. G. / 1971 / An unusual virus in cultures from a human nasopharyngeal carcinoma / J. Natl. Cancer. Inst 46 : 299 ~ 307
Brown, P. O. / 1989 / Retroviral integration: structure of the initial covalent product and its precursor, and a role for the viral IN protein / Proc. Natl. Acad. Sci. U.S.A 86 : 2
Bushman, F. D. / 1993 / Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding / Proc. Natl. Acad. Sci.
Daniel, R. / 1999 / A role for DNA-PK in retroviral DNA integration / Science 284 : 644 ~ 647
Donehower, L. A. / 1988 / Analysis of mutant Moloney murine leukemia viruses containing linker insertion mutations in the 3' region of pol / J. Virol 62 : 3958 ~ 3964
Brown, P. O. / 1989 / Retroviral integration: structure of the initial covalent product and its precursor, and a role for the viral IN protein / Proc. Natl. Acad. Sci. U.S.A 86 : 2
Bushman, F. D. / 1993 / Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding / Proc. Natl. Acad. Sci.
Daniel, R. / 1999 / A role for DNA-PK in retroviral DNA integration / Science 284 : 644 ~ 647
Donehower, L. A. / 1988 / Analysis of mutant Moloney murine leukemia viruses containing linker insertion mutations in the 3' region of pol / J. Virol 62 : 3958 ~ 3964
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