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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 256 - 261 (6page)
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초록· 키워드
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PEP-1 peptide has been used for transduction of native protein into mammalian cells. This work describes the findings that the fusion of PEP-1 to target proteins led to protein truncation likely in a non-protein-specific manner. Approximately 75% of PEP-1-MsrA fusion protein was truncated in the N-terminal region of MsrA between Lys-27 and Val-28 during expression in Escherichia coli and purification. This large protein truncation was also observed in another PEP-1 fused protein, PEP-1-MsrB2, in the N-terminal region of MsrB2. The full-length PEP-1-MsrA protein was rapidly transduced into keratinocyte cells within 15 min. The transduced PEP-1-MsrA was functionally active and could protect skin cells against oxidative stressand ultraviolet radiation-induced cell death. Collectively, our data demonstrated the protective roles of MsrA in skin cells and, moreover, may raise a concern of protein truncation caused by fusion of PEP-1 about the general use of this peptide for protein transduction. [BMB reports 2011; 44(4): 256-261]
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참고문헌 (18)
참고문헌 신청
An, J. J / 2008 / Transduced human PEP-1-heat shock protein 27 efficiently protects against brain ischemic insult / FEBS JOURNAL 275 : 1296 ~ 1308
Cabreiro, F / 2008 / Overexpression of mitochondrial methionine sulfoxide reductase B2 protects leukemia cells from oxidative stress-induced cell death and protein damage / J
Choi, H. S / 2006 / PEP-1-SOD fusion protein efficiently protects against paraquat-induced dopaminergic neuron damage in a Parkinson disease mouse model / Free Radic. Biol. M
Jung, B / 2003 / Increased viability of PC12 cells exposed to amyloid-beta peptide by transduction with human TAT-methionine sulfoxide reductase / NEUROREPORT 14 : 2349 ~ 23
Kantorow, M / 2004 / Methionine sulfoxide reductase A is important for lens cell viability and resistance to oxidative stress / Proc. Natl. Acad. Sci. U.S.A 101 : 9654 ~ 9659
Cabreiro, F / 2008 / Overexpression of mitochondrial methionine sulfoxide reductase B2 protects leukemia cells from oxidative stress-induced cell death and protein damage / J
Choi, H. S / 2006 / PEP-1-SOD fusion protein efficiently protects against paraquat-induced dopaminergic neuron damage in a Parkinson disease mouse model / Free Radic. Biol. M
Jung, B / 2003 / Increased viability of PC12 cells exposed to amyloid-beta peptide by transduction with human TAT-methionine sulfoxide reductase / NEUROREPORT 14 : 2349 ~ 23
Kantorow, M / 2004 / Methionine sulfoxide reductase A is important for lens cell viability and resistance to oxidative stress / Proc. Natl. Acad. Sci. U.S.A 101 : 9654 ~ 9659
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