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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 631 - 636 (6page)
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Aurora-A is a centrosome-localized serine/threonine kinasethat is overexpressed in multiple human cancers. We previouslyreported an intramolecular inhibitory regulation ofAurora-A between its N-terminal regulatory domain (Nt, aminoacids [aa] 1-128) and the C-terminal catalytic domain (Cd, aa129-403). Here, we demonstrate that although both Aurora-Amutants (AurA-K250G and AurA-D294G/Y295G) lackedinteractions between the Nt and Cd, they also failed to interactwith Ajuba, an essential activator of Aurora-A, leading to lossof kinase activity. Additionally, overexpression of either of themutants resulted in centrosome amplification and mitoticspindle formation defects. Both mutants were also able tocause G2/M arrest and apoptosis. These results indicate thatboth K250 and D294/Y295 are critical for direct interactionbetween Aurora-A and Ajuba and the function of the Aurora-Acomplex in cell cycle progression.
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참고문헌 (18)
참고문헌 신청
Bischoff, J. R. / 1998 / A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers / EMBO J 17 : 3052 ~ 3065
Cheetham, G. M. / 2002 / Crystal structure of Aurora-2, an oncogenic serine/threonine kinase / J. Biol. Chem 277 : 42419 ~ 42422
Du, J. / 2004 / Suppression of p160ROCK bypasses cell cycle arrest after Aurora-A/ STK15 depletion / Proc. Natl. Acad. Sci. U.S.A 101 : 8975 ~ 8980
Giet, R. / 1999 / Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases / J. Cell Sci 112 : 3591 ~ 3601
Giet, R. / 2002 / Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules / J. Cell Biol 156 : 437 ~ 451
Cheetham, G. M. / 2002 / Crystal structure of Aurora-2, an oncogenic serine/threonine kinase / J. Biol. Chem 277 : 42419 ~ 42422
Du, J. / 2004 / Suppression of p160ROCK bypasses cell cycle arrest after Aurora-A/ STK15 depletion / Proc. Natl. Acad. Sci. U.S.A 101 : 8975 ~ 8980
Giet, R. / 1999 / Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases / J. Cell Sci 112 : 3591 ~ 3601
Giet, R. / 2002 / Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules / J. Cell Biol 156 : 437 ~ 451
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