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학술저널
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대한생화학·분자생물학회 BMB Reports BMB Reports 제45권 제9호
발행연도
2012.1
수록면
515 - 520 (6page)

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High expression of osmotically responsive genes1 (HOS1), a key regulator of low temperature response and flowering time,encodes an E3 ubiquitin ligase in Arabidopsis. Here, we report characterization of a newly identified splice variant (HOS1-L)of HOS1. Comparative analyses revealed that HOS1-L has a longer 5' nucleotide sequence than that of the previously identified HOS1 (HOS1-S) and that its protein sequence was more conserved than that of HOS1-S in plants. HOS1-L transcripts were spatio-temporally more abundant than those of HOS1-S. The recovery rate of HOS1-S expression was faster than that of HOS1-L after cold treatment. Diurnal oscillation patterns of HOS1-L revealed that HOS1-L expression was affected by photoperiod. An in vitro pull-down assay revealed that the HOS1-L protein interacted with the ICE1 protein. HOS1-L overexpression caused delayed flowering in wild-type plants. Collectively, these results suggest regulation of HOS1expression at the post-transcriptional level. [BMB Reports 2012; 45(9): 515-520]

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