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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 205 - 211 (7page)
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Sprouty (Spry) proteins have previously been suggested as negative regulators of the MAPK pathway through interaction with Raf-1. However, the molecular basis of this inhibition has not been elucidated. In this study, we used cells expressing FLAGtagged Raf-1 with point mutations at known phosphorylation sites to reveal that activation of Raf-1 mutants does not correlate with their degree of interaction with Spry2. The association of Raf-1 with Spry2 in intact cells was further corroborated by immunofluorescence colocalization. Additionally, there was no significant change observed in the strength of interaction between Raf-1 mutants and Spry2 after paclitaxel treatment despite differences in the activation levels of these mutants. Thus,our study provides the evidence that Spry2 does not directly regulate Raf-1 kinase activity, but instead acts as a scaffolding protein that assists interactions between Raf-1 kinase and its direct regulators.
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참고문헌 (23)
참고문헌 신청
Ahn, J. H. / 2009 / The enhancement of Raf-1 kinase activity by knockdown of Spry2 is associated with high sensitivity to paclitaxel in v-Ha-ras-transformed NIH 3T3 fibrobla
Blagosklonny, M. V. / 1997 / Raf-1/bcl-2 phosphorylation: a step from microtubule damage to cell death / Cancer Res. 57 : 130 ~ 135
Chandramouli, S. / 2008 / Tesk1 interacts with Spry2 to abrogate its inhibitio
Cheung, H. W. / 2004 / Id-1-induced Raf/MEK pathway activation is essential for its protective role against taxol-induced apoptosis in nasopharyngeal carcinoma cells / Carci
Dhillon, A. S. / 2002 / Untying the regulation of the Raf-1 kinase / Arch. Biochem. Biophys. 404 : 3 ~ 9
Blagosklonny, M. V. / 1997 / Raf-1/bcl-2 phosphorylation: a step from microtubule damage to cell death / Cancer Res. 57 : 130 ~ 135
Chandramouli, S. / 2008 / Tesk1 interacts with Spry2 to abrogate its inhibitio
Cheung, H. W. / 2004 / Id-1-induced Raf/MEK pathway activation is essential for its protective role against taxol-induced apoptosis in nasopharyngeal carcinoma cells / Carci
Dhillon, A. S. / 2002 / Untying the regulation of the Raf-1 kinase / Arch. Biochem. Biophys. 404 : 3 ~ 9
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