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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 489 - 494 (6page)
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초록· 키워드
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The in vitro antibody discovery technologies revolutionized the generation of target-specific antibodies that traditionally relied on the humoral response of immunized animals. An antibody library, a large collection of diverse, pre-constructed antibodies, can be rapidly screened using in vitro display technologies such as phage display. One of the keys to successful in vitro antibody discovery is the quality of the library diversity. Antibody diversity can be obtained either from natural B-cell sources or by the synthetic methods that combinatorially generate random nucleotide sequences. While the functionality of a natural antibody library depends largely upon the library size, various other factors can affect the quality of a synthetic antibody library, making the design and construction of synthetic antibody libraries complicated and challenging. In this review, we present various library designs and diversification methods for synthetic antibody library.
From simple degenerate oligonucleotide synthesis to trinucleotide synthesis to physicochemically optimized library design, the synthetic approach is evolving beyond the simple emulation of natural antibodies, into a highly sophisticated method that is capable of producing high quality antibodies suitable for therapeutic, diagnostic, and other demanding applications.
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참고문헌 (23)
참고문헌 신청
Bradbury AR / 2011 / Beyond natural antibodies: the power of in vitro display technologies / Nat Biotechnol 29 : 245 ~ 254
Desiderio A / 2001 / A semi-synthetic repertoire of intrinsically stable antibody fragments derived from a single-framework scaffold / J Mol Biol 310 : 603 ~ 615
Hudson PJ / 1999 / High avidity scFv multimers;diabodies and triabodies / J Immunol Methods 231 : 177 ~ 189
Hye Young Yang / 2009 / Construction of a Large Synthetic Human scFv Library with Six Diversified CDRs and High Functional Diversity / Molecules and Cells 27 (2) : 225 ~ 235
Kim, Yu-Jin / 2008 / Improving the Productivity of Single-Chain Fv Antibody Against c-Met byRearranging the Order of its Variable Domains / Journal of Microbiology and Biote
Desiderio A / 2001 / A semi-synthetic repertoire of intrinsically stable antibody fragments derived from a single-framework scaffold / J Mol Biol 310 : 603 ~ 615
Hudson PJ / 1999 / High avidity scFv multimers;diabodies and triabodies / J Immunol Methods 231 : 177 ~ 189
Hye Young Yang / 2009 / Construction of a Large Synthetic Human scFv Library with Six Diversified CDRs and High Functional Diversity / Molecules and Cells 27 (2) : 225 ~ 235
Kim, Yu-Jin / 2008 / Improving the Productivity of Single-Chain Fv Antibody Against c-Met byRearranging the Order of its Variable Domains / Journal of Microbiology and Biote
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