지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 86 - 91 (6page)
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초록· 키워드
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Tissue-specific gene expression is regulated by epigenetic modification involving trans-acting factors. Here, we identifiedthat the human MAGEB16 gene and its mouse homolog,Mageb16, are only expressed in the testis. To investigate themechanism governing their expression, the promoter methylationstatus of these genes was examined in different samples.
Two CpG islands (CGIs) in the 5' upstream region of MAGEB16were highly demethylated in human testes, whereas they weremethylated in cells without MAGEB16 expression. Similarly, theCGI in Mageb16 was hypomethylated in mouse testes buthypermethylated in other tissues and cells without Mageb16expression. Additionally, the expression of these genes could beactivated by treatment with the demethylation agent 5'-aza-2'-deoxycytidine (5'-aza-CdR). Luciferase assays revealed that bothgene promoter activities were inhibited by methylation of theCGI regions. Therefore, we propose that the testis-specificexpression of MAGEB16 and Mageb16 is regulated by themethylation status of their promoter regions.
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참고문헌 (20)
참고문헌 신청
Chomez, P. / 2001 / An overview of the MAGE gene family with the identification of all human members of the family / Cancer Res 61 : 5544 ~ 5551
De Smet, C. / 1999 / DNA methylation is the primary silencing mechanism for a set of germ line- and tumor-specific genes with a CpG-rich promoter / Mol. Cell. Biol 19 : 7327
De Smet, C. / 2004 / Promoter-dependent mechanism leading to selective hypomethylation within the 5' region of gene MAGE-A1 in tumor cells / Mol. Cell. Biol 24 : 4781 ~ 4790
Dong, X. / 2004 / Zinc- finger protein ZNF165 is a novel cancer-testis antigen capable of eliciting antibody response in hepatocellular carcinoma patients / Br. J. Cancer 91
Gang Bai / 2010 / Promoter demethylation mediates the expression of ZNF645, a novel cancer/testis gene / BMB Reports 43 (6) : 400 ~ 406
De Smet, C. / 1999 / DNA methylation is the primary silencing mechanism for a set of germ line- and tumor-specific genes with a CpG-rich promoter / Mol. Cell. Biol 19 : 7327
De Smet, C. / 2004 / Promoter-dependent mechanism leading to selective hypomethylation within the 5' region of gene MAGE-A1 in tumor cells / Mol. Cell. Biol 24 : 4781 ~ 4790
Dong, X. / 2004 / Zinc- finger protein ZNF165 is a novel cancer-testis antigen capable of eliciting antibody response in hepatocellular carcinoma patients / Br. J. Cancer 91
Gang Bai / 2010 / Promoter demethylation mediates the expression of ZNF645, a novel cancer/testis gene / BMB Reports 43 (6) : 400 ~ 406
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