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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 14 - 19 (6page)
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A feruloyl esterase encoding gene (designated fae6), derived from a leachate metagenomic library, was cloned and the nucleotide sequence of the insert DNA determined. Translational analysis revealed that fae6 consists of a 515 amino acid polypeptide,encoding a 55 kDa pre-protein. The Fae6 primary structure contained the G-E-S-A-G sequence, which corresponds well with a typical catalytic serine sequence motif (G-x-S-x-G). The fae6 gene was successfully over-expressed in E. coli and the recombinant protein was purified to 8.4 fold enrichment with 17% recovery. The KM data showed Fae6 has a high affinity to methyl sinapate while thermostability data indicated that fae6 was thermolabile with a half life (T1/2) ＜ 30min at 50oC. High affinity for Fae6 against methyl sinapate,methyl ferulate and ethyl ferulate suggest that the enzyme can be useful in hydrolyzing ferulated polysaccharides in a biorefinery process. [BMB reports 2012; 45(1): 14-19]
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참고문헌 (37)
참고문헌 신청
Kang, C. / 2011 / A novel family VII esterase with industrial potential from compost metagenomic library / Microbial Cell Factories 10(0):0~0
Arpigny, K. L. / 1999 / Bacterial lipolytic enzymes: classification and properties / J. Biochem. 343:177~183
Hermoso, J. A. / 2004 / The crystal structure of feruloyl esterase A from Aspergillus niger suggest evolutive functional convergence in feruloyl esterase family / J. Mol. Biol. 338:495~506
Tarbouriech, N. / 2005 / Molecular determinants of substrate specificity in theferuloyl esterase module of xylanase 10B from Clostridiumthermocellum. / Acta. Crystallogr. D. Biol. Crystallogr. 61:194~197
Kouker, G. / 1987 / Specific and sensitive plate assay for bacterial lipases / Appl. Environ. Microbiol. 53:211~213
Arpigny, K. L. / 1999 / Bacterial lipolytic enzymes: classification and properties / J. Biochem. 343:177~183
Hermoso, J. A. / 2004 / The crystal structure of feruloyl esterase A from Aspergillus niger suggest evolutive functional convergence in feruloyl esterase family / J. Mol. Biol. 338:495~506
Tarbouriech, N. / 2005 / Molecular determinants of substrate specificity in theferuloyl esterase module of xylanase 10B from Clostridiumthermocellum. / Acta. Crystallogr. D. Biol. Crystallogr. 61:194~197
Kouker, G. / 1987 / Specific and sensitive plate assay for bacterial lipases / Appl. Environ. Microbiol. 53:211~213
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