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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 26 - 31 (6page)
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TRIM72 is known to play a critical role in skeletal muscle membrane repair. To better understand the molecular mechanisms of this protein, we carried out an in vitro binding study with TRIM72. Our study proved that TRIM72 binds various lipids with dissociation constants (Kd) ranging from 88.2 ± 9.9 nM to 550.5± 134.5 nM. In addition, the intrinsic fluorescence of TRIM72exponentially decreased when the protein was diluted with stirring. The time-resolved fluorescence decay occurred in a concentration-independent manner. The fluorescence-decayed TRIM72 remained in its secondary structure, but its binding properties were significantly reduced. The dissociation constants (Kd)of fluorescence-decayed TRIM72 for palmitate and stearate were 159.1 ± 39.9 nM and 355.4 ± 106.0 nM, respectively. This study suggests that TRIM72 can be dynamically converted by various stimuli. The results of this study also provide insight into the role of TRIM72 in the repair of sarcolemma damage. (BMB reports 2012; 45(1): 26-31)
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참고문헌 (11)
참고문헌 신청
Cai, C. / 2009 / MG53 regulates membrane budding and exocytosis in muscle cells / J. Biol. Chem. 284 : 3314 ~ 3322
Cai, C. / 2009 / Membrane repair defects in muscular dystrophy are linked to altered interaction between MG53, caveolin-3, and dysferlin / J. Biol. Chem. 284 : 15894 ~ 15902
Cai, C. M. H. / 2009 / MG53 nucleates assembly of cell membrane repair machinery / Nat. Cell Biol. 11 : 55 ~ 64
Hwang, M. / 2011 / Redox-dependent oligomerization through a leucine zipper motif is essential for MG53-mediated cell membrane repair / Am. J. Physiol. Cell Physiol. 301 : 1
Jung, S. Y. and Ko, Y. G. / 2010 / TRIM72, a novel negative feedback regulator of myogenesis, is transcriptionally activated by the synergism of MyoD (or myogenin) and MEF2 /
Cai, C. / 2009 / Membrane repair defects in muscular dystrophy are linked to altered interaction between MG53, caveolin-3, and dysferlin / J. Biol. Chem. 284 : 15894 ~ 15902
Cai, C. M. H. / 2009 / MG53 nucleates assembly of cell membrane repair machinery / Nat. Cell Biol. 11 : 55 ~ 64
Hwang, M. / 2011 / Redox-dependent oligomerization through a leucine zipper motif is essential for MG53-mediated cell membrane repair / Am. J. Physiol. Cell Physiol. 301 : 1
Jung, S. Y. and Ko, Y. G. / 2010 / TRIM72, a novel negative feedback regulator of myogenesis, is transcriptionally activated by the synergism of MyoD (or myogenin) and MEF2 /
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