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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 692 - 697 (6page)
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To investigate the pathways of oxidoreductases in plants, 2 key components in thioredox systems i.e. thioredoxin h (Trx h) and NADPH-dependent thioredoxin reductase (NTR) genes were first isolated from tomatoes (Solanum lycopersicum). Subsequently,the coding sequences of Trx h and NTR were inserted into pET expression vectors, and overexpressed in Escherichia coli. In the UV-Visible spectra of the purified proteins, tomato Trx h was shown to have a characteristic ‘shoulder’ at ∼290nm, while the NTR protein had the 3 typical peaks unique to flavoenzymes. The activities of both proteins were demonstrated by following insulin reduction, as well as DTNB reduction. Moreover, both NADPH and NADH could serve as substrates in the NTR reduction system, but the catalytic efficiency of NTR with NADPH was 2500-fold higher than with NADH. Additionally, our results reveal that the tomato Trx system might be involved in oxidative stress, but not in cold damage. [BMB reports 2011; 44(10): 692-697]
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참고문헌 (17)
참고문헌 신청
Arner, E. S / 2000 / Physiological functions of thioredoxin and thioredoxin reductase / Eur. J. Biochem 267 : 6102 ~ 6109
Bashandy, T / 2010 / Interplay between the NADP-linked thioredoxin and glutathione systems in Arabidopsis auxin signaling / Plant Cell 22 : 376 ~ 391
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Carmel-Harel, O / 2000 / Roles of the glutathione- and thioredoxin-dependent reduction systems in the Escherichia coli and Saccharomyces cerevisiae responses to oxidative st
Changbo Dai / 2010 / Expression Pattern of a Peptide Methionine Sulfoxide Reductase Gene from Tomato (Solanum lycopersicum) in Response to Abiotic and Oxidative Stresses / J
Bashandy, T / 2010 / Interplay between the NADP-linked thioredoxin and glutathione systems in Arabidopsis auxin signaling / Plant Cell 22 : 376 ~ 391
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Carmel-Harel, O / 2000 / Roles of the glutathione- and thioredoxin-dependent reduction systems in the Escherichia coli and Saccharomyces cerevisiae responses to oxidative st
Changbo Dai / 2010 / Expression Pattern of a Peptide Methionine Sulfoxide Reductase Gene from Tomato (Solanum lycopersicum) in Response to Abiotic and Oxidative Stresses / J
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