지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 175 - 180 (6page)
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초록· 키워드
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Cyt2Aa2 is a mosquito larvicidal and cytolytic toxin produced by Bacillus thuringiensis subsp. darmstadiensis. The toxin becomes inactive when isoleucine at position 150 was replaced by alanine. To investigate the functional role of this position,Ile150 was substituted with Leu, Phe, Glu and Lys. All mutant proteins were produced at high level, solubilized in carbonate buffer and yielded protease activated product similar to those of the wild type. Intrinsic fluorescence spectra analysis suggested that these mutants retain similar folding to the wild type. However, mosquito larvicidal and hemolytic activities dramatically decreased for the I150K and were completely abolished for I150A and I150F mutants. Membrane binding and oligomerization assays demonstrated that only I150E and I150L could bind and form oligomers on lipid membrane similar to that of the wild type. Our results suggest that amino acid at position 150 plays an important role during membrane binding and oligomerization of Cyt2Aa2 toxin.
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참고문헌 (23)
참고문헌 신청
Butko, P. / 2003 / Cytolytic toxin Cyt1A and its mechanism of membrane damage: data and hypotheses / Appl. Environ. Microbiol 69 : 2415 ~ 2422
Cohen, S. / 2008 / High-resolution crystal structure of activated Cyt2Ba monomer from Bacillus thuringiensis subsp. israelensis / J. Mol. Biol 380 : 820 ~ 827
Cohen, S. / 2011 / Cyt1Aa toxin: crystal structure reveals implications for its membrane-perforating function / J. Mol. Biol 413 : 804 ~ 814
Crickmore, N. / 1998 / Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins / Microbiol. Mol. Biol. Rev 62 : 807 ~ 813
Finney, D. / 1971 / Probit Analysis / Cambridge University Press
Cohen, S. / 2008 / High-resolution crystal structure of activated Cyt2Ba monomer from Bacillus thuringiensis subsp. israelensis / J. Mol. Biol 380 : 820 ~ 827
Cohen, S. / 2011 / Cyt1Aa toxin: crystal structure reveals implications for its membrane-perforating function / J. Mol. Biol 413 : 804 ~ 814
Crickmore, N. / 1998 / Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins / Microbiol. Mol. Biol. Rev 62 : 807 ~ 813
Finney, D. / 1971 / Probit Analysis / Cambridge University Press
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