지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 159 - 164 (6page)
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초록· 키워드
오류제보하기
CD99 is known to be involved in the regulation of cell-cell adhesion. However, it remains unclear whether CD99 controls cell-extracellular matrix adhesion. In this study, the effects of CD99 activation on cell-extracellular matrix adhesion were investigated. It was found that engagement of CD99 with the stimulating antibody YG32 downregulated the adhesion of MCF-7 cells to fibronectin, laminin and collagen IV in a dose-dependent manner. The CD99 effect on cell-ECM adhesion was inhibited by overexpression of the dominant negative form of CD99 or CD99 siRNA transfection. Treatment of cells with Mn2+ or by β1 integrin-stimulating antibody restored the inhibitory effect of CD99 on cell-ECM adhesion. Cross-linking CD99 inactivated β1 integrin through conformational change.
CD99 activation caused dephosphorylation at Tyr-397 in FAK,which was restored by the β1 stimulating antibody. Taken together,these results provide the first evidence that CD99 inhibits cell-extracellular matrix adhesion by suppressing β1 integrin affinity. [BMB reports 2012; 45(3): 159-164]
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Al-Jamal, R. / 2008 / Beta1 integrin in tissue remodelling and repair: from phenomena to concepts / Pharmacol. Ther. 120 : 81 ~ 101
Alberti, I. / 2002 / CD99 isoforms expression dictates T cell functional outcomes / FASEB J. 16 : 1946 ~ 1948
Bernard, G. / 2000 / CD99 (E2) up-regulates alpha4beta1-dependent T cell adhesion to inflamed vascular endothelium under flow conditions / Eur. J. Immunol. 30 : 3061 ~ 3065
Dustin, M. L. / 1989 / T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1 / Nature 341 : 619 ~ 624
Fenczik, C. A. / 1997 / Complementation of dominant suppression implicates CD98 in integrin activation / Nature 390 : 81 ~ 85
Alberti, I. / 2002 / CD99 isoforms expression dictates T cell functional outcomes / FASEB J. 16 : 1946 ~ 1948
Bernard, G. / 2000 / CD99 (E2) up-regulates alpha4beta1-dependent T cell adhesion to inflamed vascular endothelium under flow conditions / Eur. J. Immunol. 30 : 3061 ~ 3065
Dustin, M. L. / 1989 / T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1 / Nature 341 : 619 ~ 624
Fenczik, C. A. / 1997 / Complementation of dominant suppression implicates CD98 in integrin activation / Nature 390 : 81 ~ 85
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