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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 546 - 551 (6page)
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The insecticidal activity of Bacillus thuringiensis (Bt) Cry toxinsinvolves toxin stabilization, oligomerization, passage acrossthe peritrophic membrane (PM), binding to midgut receptorsand pore-formation. The residues Arg-158 and Tyr-170 havebeen shown to be crucial for the toxicity of Bt Cry4Ba. Wecharacterized the biological function of these residues. Inmosquito larvae, the mutants R158A/E/Q (R158) could hardlypenetrate the PM due to a significantly reduced ability to alterPM permeability; the mutant Y170A, however, could passthrough the PM, but degraded in the space between the PMand the midgut epithelium. Further characterization byoligomerization demonstrated that Arg-158 mutants failed toform correctly sized high-molecular weight oligomers. This isthe first report that Arg-158 plays a role in the formation ofCry4Ba oligomers, which are essential for toxin passage acrossthe PM. Tyr-170, meanwhile, is involved in toxin stabilization in the toxic mechanism of Cry4Ba in mosquito larvae.
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참고문헌 (21)
참고문헌 신청
Angsuthanasombat, C. / 1991 / Cytotoxicity of a cloned Bacillus thuringiensis subsp. israelensis CryIVB toxin to an Aedes aegypti cell line / FEMS Microbiol. Lett 67 : 273 ~
Angsuthanasombat, C. / 2004 / Bacillus thuringiensis Cry4A and Cry4B mosquito-larvicidal proteins: homology- based 3D model and implications for toxin activity / J. Biochem.
Boonserm, P. / 2005 / Crystal structure of the mosquito-larvicidal toxin Cry4Ba and its biological implications / J. Mol. Biol 348 : 363 ~ 382
Bravo, A. / 2007 / Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control / Toxicon 49 : 423 ~ 435
Gerber, D. / 2000 / Insertion and organization within membranes of the δ-endotoxin pore-forming domain, helix 4-loop-helix 5, and inhibition of its activity by a mutant heli
Angsuthanasombat, C. / 2004 / Bacillus thuringiensis Cry4A and Cry4B mosquito-larvicidal proteins: homology- based 3D model and implications for toxin activity / J. Biochem.
Boonserm, P. / 2005 / Crystal structure of the mosquito-larvicidal toxin Cry4Ba and its biological implications / J. Mol. Biol 348 : 363 ~ 382
Bravo, A. / 2007 / Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control / Toxicon 49 : 423 ~ 435
Gerber, D. / 2000 / Insertion and organization within membranes of the δ-endotoxin pore-forming domain, helix 4-loop-helix 5, and inhibition of its activity by a mutant heli
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