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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 270 - 275 (6page)
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초록· 키워드
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It is essential to analyze rare mutations in many fields of biomedical research. However, the detection of rare mutations is usually failed due to the interference of predominant wild-type DNA surrounded. Herein we describe a sensitive and facile method of detecting rare point mutation on the basis of allele-specific amplification in emulsion PCR. The identification and selective amplification of rare mutation are accomplished in one-pot reaction. The allele-specific primers coupled on magnetic beads allow the exclusive amplification and enrichment of the mutant amplicons. The productive beads bearing mutant amplicons are subsequently stained with the fluorescent dyes. Thus, the rare point mutations with a percentage as low as 0.1%, can be detected by fluorescent analysis.
The relative percentages of mutation among different samples can be roughly accessed by counting the fraction of fluorescent positive beads through flow cytometry.
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참고문헌 (22)
참고문헌 신청
Bortolin, S. / 2009 / Multiplex genotyping for thrombophilia- associated SNPs by universal bead arrays / Methods Mol. Biol 496 : 59 ~ 72
Diehl, F. / 2005 / Detection and quantification of mutations in the plasma of patients with colorectal tumors / Proc. Natl. Acad. Sci. U.S.A 102 : 16368 ~ 16373
Diehl, F. / 2006 / BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions / Nat. Methods 3 : 551 ~ 559
Diehl, F. / 2008 / Circulating mutant DNA to assess tumor dynamics / Nat. Med 14 : 985 ~ 990
Dressman, D. / 2003 / Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations / Proc. Natl. Acad. Sci. U.S.A 100
Diehl, F. / 2005 / Detection and quantification of mutations in the plasma of patients with colorectal tumors / Proc. Natl. Acad. Sci. U.S.A 102 : 16368 ~ 16373
Diehl, F. / 2006 / BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions / Nat. Methods 3 : 551 ~ 559
Diehl, F. / 2008 / Circulating mutant DNA to assess tumor dynamics / Nat. Med 14 : 985 ~ 990
Dressman, D. / 2003 / Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations / Proc. Natl. Acad. Sci. U.S.A 100
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