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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 264 - 269 (6page)
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초록· 키워드
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Pattern recognition receptors are known to participate in the activation of Prophenoloxidase system. In this study, a 1,3-β-D-glucan recognition protein was detected for the first time in Antheraea pernyi larvae (Ap-βGRP). Ap-βGRP was purified to 99.9% homogeneity from the hemolymph using traditional chromatographic methods. Ap-βGRP specifically bind 1,3-β-D-glucan and yeast, but not E. coli or M. luteus.
The 1,3-β-D-glucan dependent phenoloxidase (PO) activity of the hemolymph inhibited by anti-Ap-βGRP antibody could be recovered by addition of purified Ap-βGRP. These results demonstrate that Ap-βGRP acts as a biosensor of 1,3-β-Dglucan to trigger the Prophenoloxidase system. A trace mount of 1,3-β-D-glucan or Ap-βGRP alone was unable to trigger the proPO system, but they both did. Ap-βGRP was specifically degraded following the activation of proPO with 1,3-β-Dglucan.
These results indicate the variation in the amount of Ap-βGRP after specific immune challenge in A. pernyi hemolymph is an important regulation mechanism to immune response.
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참고문헌 (20)
참고문헌 신청
Bachman, E. S. / 1996 / Molecular cloning of the first metazoan β-1,3 glucanase from eggs of the sea urchin Strongylocentrotus purpuratus / Proc. Natl. Acad. Sci 93 : 6808 ~ 6813
Bezouska, K. / 1994 / Rat natural killer cell antigen. NKR-P1, related to C-type animal lectins is a carbohydrate-binding protein / J. Biol. Chem 2269 : 16945 ~ 16952
Fabrick, J. A. / 2003 / cDNA cloning, purification, properties, and function of a β-1,3-glucan recognition protein from a pyralid moth, Plodia interpunctella / Insect Bioch
Jiang, H. / 2004 / β-1,3-glucan recognition protein-2 (βGRP-2) from Manduca sexta; an acute-phase protein that binds β-1,3-glucan and lipoteichoic acid to aggregate fungi
Jiravanichpaisal, P. / 2006 / Cell mediated immunity in arthropods: hematopoiesis, coagulation, melanization and opsonization / Immunobiology 11 : 213 ~ 236
Bezouska, K. / 1994 / Rat natural killer cell antigen. NKR-P1, related to C-type animal lectins is a carbohydrate-binding protein / J. Biol. Chem 2269 : 16945 ~ 16952
Fabrick, J. A. / 2003 / cDNA cloning, purification, properties, and function of a β-1,3-glucan recognition protein from a pyralid moth, Plodia interpunctella / Insect Bioch
Jiang, H. / 2004 / β-1,3-glucan recognition protein-2 (βGRP-2) from Manduca sexta; an acute-phase protein that binds β-1,3-glucan and lipoteichoic acid to aggregate fungi
Jiravanichpaisal, P. / 2006 / Cell mediated immunity in arthropods: hematopoiesis, coagulation, melanization and opsonization / Immunobiology 11 : 213 ~ 236
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