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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 233 - 238 (6page)
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We present a top down separation platform for yeast ribosomal proteins using affinity chromatography and capillary electrophoresis which is designed to allow deposition of proteins onto a substrate. FLAG tagged ribosomes were affinity purified, and rRNA acid precipitation was performed on the ribosomes followed by capillary electrophoresis to separate the ribosomal proteins. Over 26 peaks were detected with excellent reproducibility (＜0.5% RSD migration time). This is the first reported separation of eukaryotic ribosomal proteins using capillary electrophoresis. The two stages in this workflow, affinity chromatography and capillary electrophoresis, share the advantages that they are fast, flexible and have small sample requirements in comparison to more commonly used techniques. This method is a remarkably quick route from cell to separation that has the potential to be coupled to high throughput readout platforms for studies of the ribosomal proteome. [BMB reports 2012; 45(4): 233-238]
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참고문헌 (24)
참고문헌 신청
[학술지
Alonso, J. / 2006 / Characterisation of the drosophila melanogaster ribosomal proteome / J. Proteome Res. 5 : 2025 ~ 2032
Ban, N. / 2000 / The complete atomic structure of the large ribosomalsubunit at 2.4 angstrom resolution / Science 289 : 905 ~ 920
Beranova-Giorgianni, S. / 2003 / Proteome analysis by two-dimensional gel electrophoresis and mass spectrometry: strengths and limitations / Trends Anal. Chem. 22 : 273 ~ 281
Cech, T. / 2000 / The ribosome is a ribozyme / Science 289 : 878 ~ 879
Alonso, J. / 2006 / Characterisation of the drosophila melanogaster ribosomal proteome / J. Proteome Res. 5 : 2025 ~ 2032
Ban, N. / 2000 / The complete atomic structure of the large ribosomalsubunit at 2.4 angstrom resolution / Science 289 : 905 ~ 920
Beranova-Giorgianni, S. / 2003 / Proteome analysis by two-dimensional gel electrophoresis and mass spectrometry: strengths and limitations / Trends Anal. Chem. 22 : 273 ~ 281
Cech, T. / 2000 / The ribosome is a ribozyme / Science 289 : 878 ~ 879
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