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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 263 - 267 (5page)
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초록· 키워드
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To characterize the biochemical properties of the PP2A regulatory B subunit, PPP2R5D, we analyzed its phosphorylation sites, stoichiometry and effect on holoenzyme activity. PPP2R5D was phosphorylated on Ser-53, Ser-68, Ser-81, and Ser-566 by protein kinase A, and mutations at all four of these sites abolished any significant phosphorylation in vitro. In HEK293cells, however, the Ser-566 was the major phosphorylation site after PKA activation by forskolin, with marginal phosphorylation on Ser-81. Inhibitory tyrosine phosphorylation on Tyr-307 of the PP2A catalytic C subunit was decreased after forskolin treatment. Kinetic analysis showed that overall PP2A activity was increased with phosphorylation by PPP2R5D phosphorylation. The apparent Km was reduced from 11.25μM to 1.175 μM with PPP2R5D phosphorylation, resulting in an increase in catalytic activity. These data suggest that PKAmediated activation of PP2A is enabled by PPP2R5D phosphorylation,which modulates the affinity of the PP2A holoenzyme to its physiological substrates.
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참고문헌 (17)
참고문헌 신청
Ahn, J. H. / 2007 / Protein kinase A activates protein phosphatase 2A by phosphorylation of the B56delta subunit / Proc. Natl. Acad. Sci. U.S.A. : 2979 ~ 2984
Ahn, J. H. / 2007 / The B''/PR72 subunit mediates Ca2+-dependent dephosphorylation of DARPP-32 by protein phosphatase 2A / Proc. Natl. Acad. Sci. U.S.A. : 9876 ~ 9881
Batut, J. / 2008 / Two highly related regulatory subunits of PP2A exert opposite effects on TGF-beta/Activin/Nodal signalling / Development 135 : 2927 ~ 2937
Bibb, J. A. / 2001 / Effects of chronic exposure to cocaine are regulated by the neuronal protein Cdk5 / Nature 410 : 376 ~ 380
Fukui, R. / 2003 / Effect of methylphenidate on dopamine/DARPP signalling in adult, but not young, mice / J. Neurochem. 87 : 1391 ~ 1401
Ahn, J. H. / 2007 / The B''/PR72 subunit mediates Ca2+-dependent dephosphorylation of DARPP-32 by protein phosphatase 2A / Proc. Natl. Acad. Sci. U.S.A. : 9876 ~ 9881
Batut, J. / 2008 / Two highly related regulatory subunits of PP2A exert opposite effects on TGF-beta/Activin/Nodal signalling / Development 135 : 2927 ~ 2937
Bibb, J. A. / 2001 / Effects of chronic exposure to cocaine are regulated by the neuronal protein Cdk5 / Nature 410 : 376 ~ 380
Fukui, R. / 2003 / Effect of methylphenidate on dopamine/DARPP signalling in adult, but not young, mice / J. Neurochem. 87 : 1391 ~ 1401
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