지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 1 - 29 (29page)
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ObjectivesDictamni Radicis Cortex extracts (DRC) has been known to suppress allergic reaction, however the cellulartarget of DRC and its mode of action remain unclear. The purpose of this study is to investigate the effects ofDictamni Radicis Cortex extracts on DNCB induced atopic dermatitis-like skin lesions of NC/Nga mouse. MethodsThis study was designed to investigate the effects of DRC extract in the DNP-IgE-induced activation of MC/9murine mast cell lines in vitro and in the DNCB-induced activation of NC/Nga mouse in vivo. For thisinvestigation, We examined IL-4, IL-5, IL-6, IL-13, TNF-α and GM-CSF mRNA expression by Real-time PCR,IL-13, MIP-1α production by ELISA analysis and manifestations of NFAT1, NFAT2, AP-1 and NF-κB p65transcription factors by western blotting in vitro. Then, we examined WBC, eosinophil and neutrophil in NC/Ngamouse, IL-5, IL-13 in serum, IFN-γ, IL-4 in the spleenocyte culture supernatant, the absolute cell numbers ofCD4+, CD8+, +Gr-1+CD11b, B220+CD23+ in the ALN, PBMCs and dorsal skin, IL-5, IL-13 in the dorsal skin byReal-time PCR and the distribution of mast cells by H&E and toluidine blue. ResultsIn vitro the mRNA expression of IL-4, IL-5, IL-6, IL-13, TNF-α, GM-CSF and IL-13, MIP-1α production byELISA analysis were completely abolished by DRC and the western blot analysis decreased the expression of mastcell-specific transcription factors including NFAT-1, NF-κB p65. In vivo DRC oral adminstration also decreasedthe counts of WBC, eosinophils and inflammatory cytokines such as IL-13 and IgE in the serum. DRC oraladminstration elevated IL-4 level in the spleenocyte culture supernatant. DRC oral adminstration decreased totalALN cells, total skin cells, cell numbers of CD4+, B220+CD23+ in the ALN, +Gr-1+CD11b in the PBMCs andCD4+, CD8+ in the dorsal skin. The mRNA expression of IL-5, IL-13, thickness of epidermis, inflammationimmune cells and mast cells were abolished by DRC in the dorsal skin. ConclusionsHistological examination showed that infiltration levels of immune cells in the skin of AD-induced NC/Ngamouse were much improved by DRC oral adminstration. These results, therefore, suggest that DRC can regulatemolecular mediators and immune cells that are functionally associated with atopic dermatitis induced in NC/Ngamouse, and may play an important role in recovering AD symptoms.
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참고문헌 (31)
참고문헌 신청
Bae GH / 2000 / Korea's medicinal plants / Gyoakssa : 122 ~
Bang CK / 2007 / The effects of BGG on various immunological factors related to pathogenesis of allergic dermatitis in NC/Nga mice induced by Derf / Daejeon University Insti
Chang IM / 2003 / Treatise on asian herbal medicines / Seoul National University Press Inc : 176 ~ 177
Fleischer AB / 1999 / Role of tacrolimus ointment as a topical noncorticosteroidal therapy / J Allergy Clin Immunol 104 (1) : 126 ~ 130
Goka AK / 1987 / The use of 3, 3', 5, 5'-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay / J Immunoassay 8 (1) : 29 ~ 41
Bang CK / 2007 / The effects of BGG on various immunological factors related to pathogenesis of allergic dermatitis in NC/Nga mice induced by Derf / Daejeon University Insti
Chang IM / 2003 / Treatise on asian herbal medicines / Seoul National University Press Inc : 176 ~ 177
Fleischer AB / 1999 / Role of tacrolimus ointment as a topical noncorticosteroidal therapy / J Allergy Clin Immunol 104 (1) : 126 ~ 130
Goka AK / 1987 / The use of 3, 3', 5, 5'-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay / J Immunoassay 8 (1) : 29 ~ 41
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