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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 188 - 196 (9page)
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Background/Aims: Epigallocatechin-3-gallate (EGCG), the primary catechin in green tea, has anti-inflammatory and anti-oxidative properties. The aim of the current study was to characterize the impact of EGCG on lipopolysaccharide (LPS)-induced innate signaling in bone marrow-derived macrophages (BMMs) isolated from ICR mice. Methods: The effect of EGCG on LPS-induced pro-infl ammatory gene expression and nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling was examined using reverse transcription-polymerase chain reaction, Western blotting,immunofl uorescence, and the electrophoretic mobility shift assay. Results: EGCG inhibited accumulation of LPS-induced IL-12p40, IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA in BMMs.
EGCG blocked LPS-induced IκBα degradation and RelA nuclear translocation. EGCG blocked the DNA-binding activity of NF-κB. LPS-induced phosphorylation of ERK1/2, JNK,and p38 was inhibited by EGCG. U0126 (an inhibitor of MEK-1/2) suppressed the LPS-induced IL-12p40, IL-6, MCP-1,ICAM-1, and VCAM-1 mRNA accumulation in BMMs. Conclusions:These results indicate that EGCG may prevent LPSinduced pro-infl ammatory gene expression through blocking NF-κB and MAPK signaling pathways in BMMs.
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참고문헌 (46)
참고문헌 신청
Liu J / 2010 / Regulation of lipopolysaccharideinduced inflammatory response by heat shock protein 27 in THP-1 cells / Cell Immunol 264:127~134
Sánchez-Fidalgo S / 2010 / Cpolysaccharide-induced inflammatory response by heat shock protein 27 in THP-1 cells. Cell Immunol 2010;264:127-134. lemmmation in mice / Eur J Pharmacol 633:78~84
Sheng KC / 2010 / Reactive oxygen species level defines two functionally distinctive stages of inflammatory dendritic cell development from mouse bone marrow / J Immunol 184:2863~2872
Chambers TJ / 1993 / Generation of osteoclast-inductive and osteoclastogenic cell lines from the H-2KbtsA58 transgenic mouse / Proc Natl Acad Sci U S A 90:5578~5582
Osborn L / 1989 / Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B / Proc Natl Acad Sci U S A 86:2336~2340
Sánchez-Fidalgo S / 2010 / Cpolysaccharide-induced inflammatory response by heat shock protein 27 in THP-1 cells. Cell Immunol 2010;264:127-134. lemmmation in mice / Eur J Pharmacol 633:78~84
Sheng KC / 2010 / Reactive oxygen species level defines two functionally distinctive stages of inflammatory dendritic cell development from mouse bone marrow / J Immunol 184:2863~2872
Chambers TJ / 1993 / Generation of osteoclast-inductive and osteoclastogenic cell lines from the H-2KbtsA58 transgenic mouse / Proc Natl Acad Sci U S A 90:5578~5582
Osborn L / 1989 / Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B / Proc Natl Acad Sci U S A 86:2336~2340
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