지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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To elucidate the cytotoxicity and antioxidative effect of Portulaca oleracea L. (PO) extract on copper (Cu), an allergic contact dermatitis(ACD)-induced agent, cell viability were analysed by XTT assay after the treatment of cupric sulfate (CuSO4) to cultured human skin fibroblasts (Detroit 551). And also, the effects of vitamin E and PO extract on CuSO4-induced cytotoxicity were measured after Detroit 551 cells were pretreated with vitamin E or PO extract for 2 hours. In the antioxidative effects of PO extract were measured by superoxide dismutase (SOD)-like activity, the inhibitory activity of lipid peroxidation (LP) and lactate dehydrogenase (LDH) activity. In this study, CuSO4 significantly decreased cell viability in dose-dependently compared with control, and the XTT50 value was calculated at 59.8 μM of CuSO4. While, vitamin E effectively prevented CuSO4-induced cytotoxicity and also, PO extract significantly increased cell viability compared with CuSO4-treated group. In the antioxidative effect, PO extract showed the inhibitory activity of LP and the decrease of LDH activity as well as SOD-like activity. From these results, it is suggested that the PO extract effectively prevented the cytotoxicity induced by an ACD-induced agent, CuSO4 via antioxidative effect. Conclusively, plant extract such as PO may be a putative resources as an alternative antioxidant for the prevention or diminution of ACD induced by allergen of heavy metals correlated with oxidative stress.
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참고문헌 (28)
참고문헌 신청
Batista BL / 2008 / Simultaneous determination of Cd, Cu, Mn, Ni, Pb and Zn in nail samples by inductively coupled plasma mass spectrometry (ICP-MS) after tetramethylammoniu
Chen CY / 2001 / Effects of nickel chloride on human platelets: enhancement of lipid peroxidation, inhibition of aggregation and interaction with ascorbic acid / J Toxicol E
Counts AL / 2002 / Nickel allergy associated with a transpalatal arch appliance / J Orofac Orthop 63 (6) : 509 ~ 515
Goodman JR / 1969 / Role of copper in iron localization in developing erythrocyte / Blood 6 : 747 ~ 755
Hall E / 1986 / Role of lipid peroxidation in post-traumatic spinal cord degeneration / CNS Trauma 3 : 281 ~ 294
Chen CY / 2001 / Effects of nickel chloride on human platelets: enhancement of lipid peroxidation, inhibition of aggregation and interaction with ascorbic acid / J Toxicol E
Counts AL / 2002 / Nickel allergy associated with a transpalatal arch appliance / J Orofac Orthop 63 (6) : 509 ~ 515
Goodman JR / 1969 / Role of copper in iron localization in developing erythrocyte / Blood 6 : 747 ~ 755
Hall E / 1986 / Role of lipid peroxidation in post-traumatic spinal cord degeneration / CNS Trauma 3 : 281 ~ 294
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