지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
To clarify the cytotoxicity and antioxidative effect of Solanum nigrum L. (SN) extract, cell adhesion activity and cell viability were analysed by XTT or MTT assay after human dermal fibroblasts (Detroit 551) were cultured in media containing various concentrations of H2O2. And also, the effect of antioxidant, butylated hydroxytoluene (BHT), against H2O2-induced cytotoxicity was examined. For the protective effect of SN extract on H2O2-induced cytotoxicity, Detroit 551 cells were pretreated with 40 or 60 μg/mL of SN extract for 2 hours. And also, the antioxidative effects of SN extract were measured by superoxide dismutase (SOD)-like activity and the inhibitory activity of lipid peroxidation. In this study, H2O2 remarkably decreased cell viability as well as cell adhesion activity in dose-dependent manner compared with control. And the XTT50 and MTT50 values were determined at 35 and 40 μM of H2O2, respectively. In the effect of antioxidant, BHT effectively prevented H2O2-induced cytotoxicity by the significant increase in cell adhesion activity. In the protective effect of SN extract on H2O2-induced cytotoxicity, SN extract remarkably increased cell adhesion activity which was decreased by H2O2-induced cytotoxicity, and also it showed both of SOD-like activity and the inhibitory activity of lipid peroxidation. From these results,it is suggested that the SN extract effectively prevented the cytotoxicity induced by an oxidant, H2O2 as component of oxidized hair dye via its antioxidative effect, and also it may be a putative resources for beauty as an alternative antioxidant in hair dye.
목차
등록된 정보가 없습니다.
참고문헌 (34)
참고문헌 신청
[학술지
Arditi T / 2000 / Renewed interest in soy isoflavones and saponins / Cereal Foods World 45 : 414 ~ 417
Boczkowski J / 1999 / Endogenous peroxynitrite mediates mitochondrial dysfunction in rat diaphragm during endotoxemia / FASEB J 13 : 1637 ~ 1646
Bohm F / 1997 / Carotinoids enhance vitamin E antioxidant efficiency / J Am Chem Soc 119 : 621 ~ 622
Borenfreund E / 1984 / A simple quantitative procedure using monolayer culture for cytotoxicity assay (HTD/NR-90) / J Tiss Cult Meth 9 : 7 ~ 9
Arditi T / 2000 / Renewed interest in soy isoflavones and saponins / Cereal Foods World 45 : 414 ~ 417
Boczkowski J / 1999 / Endogenous peroxynitrite mediates mitochondrial dysfunction in rat diaphragm during endotoxemia / FASEB J 13 : 1637 ~ 1646
Bohm F / 1997 / Carotinoids enhance vitamin E antioxidant efficiency / J Am Chem Soc 119 : 621 ~ 622
Borenfreund E / 1984 / A simple quantitative procedure using monolayer culture for cytotoxicity assay (HTD/NR-90) / J Tiss Cult Meth 9 : 7 ~ 9
이 논문의 저자 정보
최근 본 자료
댓글(0)
0