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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Serum plays a role as biomarker in the assessment of health condition or severity of diseases. The separation method, storage temperature and duration affect the levels of cytokines and its biochemical constituents. In this study, blood sampling was performed from Hanwoo cattle through jugular venipuncture. All blood samples were kept at 26±3℃ for 1~2 h and then separated into four conditions (groups a-d). For group (a), the serum was immediately separated (control group) and stored at -70℃; for group (b) the separated serum was kept in ice box for 2 h and then stored at -70℃; for group (c) the collected blood samples were stored in ice box for 2hrs before serum separation; and for group (d); the whole blood was kept at 4℃ for 24hrs before serum separation and then stored at -70℃. All serum samples were stored at -70℃ until analysis. Depending on serum separation time, storage period and temperature, IFN-γ level revealed no significant differences; whereas, TNF-α level was significantly lower (p<0.05) in group (d) than in groups (a-c). Besides, concentration of albumin and total protein were significantly higher (p<0.01) in (d) than that in group (a). The levels of magnesium and calcium also echoed similar results (p<0.05). But G-GTP was significantly lower (p<0.05) in group (d) compared with other groups. Therefore, considering the distance between the farm and the laboratory, it is necessary to minimize the effect of temperature and storage time on serum cytokine and biochemical concentration.
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등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Arend, W. P. / 1995 / Inhibition of the production and effects of interleukin-1 and tumor necrosis factor alpha in rheumatoid arthritis / Arthritis Rheum 38:151~160
Aziz, N. / 1999 / Variables that affect assays for plasma cytokines and soluble activation markers / Clin. Diagn. Lab. Immunol 6:89~95
Bose, S. / 2003 / Temporal activation of NF-κB regulates an interferon-independent innate antiviral response against cytoplasmic RNA viruses / Proc. Natl. Acad. Sci. USA 100:10890~10895
Clark, S. / 2003 / Stability of plasma analytes after delayed separation of whole blood:implications for epidemiological studies / Int. J. Epidemiol 32:125~130
Ehsani, A. / 2008 / Serum constituents analyses in dairy cows: effects of duration and temperature of the storage of clotted blood / Res. Vet. Sci 85:473~475
Aziz, N. / 1999 / Variables that affect assays for plasma cytokines and soluble activation markers / Clin. Diagn. Lab. Immunol 6:89~95
Bose, S. / 2003 / Temporal activation of NF-κB regulates an interferon-independent innate antiviral response against cytoplasmic RNA viruses / Proc. Natl. Acad. Sci. USA 100:10890~10895
Clark, S. / 2003 / Stability of plasma analytes after delayed separation of whole blood:implications for epidemiological studies / Int. J. Epidemiol 32:125~130
Ehsani, A. / 2008 / Serum constituents analyses in dairy cows: effects of duration and temperature of the storage of clotted blood / Res. Vet. Sci 85:473~475
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