지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Although it has been reported that lysyl oxidase (LOX) is involved in odontoblastic differentiation, the role of LOX on odontoblastic differentiation by hydrogen peroxide (H2O2) have not been clarified. In the present study, we investigated whether H2O2, reactive oxygen species (ROS), is modulated the messenger RNA (mRNA) expression and activity of LOX during odontoblastic differentiation of human dental pulp (HDP) cells. The mRNA expression was quantified by reverse transcriptase polymerase chain reaction (RT-PCR) analysis, and LOX enzyme activity was measured by high sensitive fluorescent assay. Expression of the odontoblastic differentiation marker genes were assessed in the presence and absence of specific small interfering RNAs (siRNAs) of the LOX and LOXL. The H2O2-induced mRNA expression of LOX family was significant reduction of LOX,LOXL, and LOXL3 mRNA levels in HDP cells. LOX enzyme activity was increased at H2O2 0.3 mM for 24 hours. The mRNA expression of alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN) was inhibited by LOX- and LOXL-specific siRNAs whereas the mRNA expression of dentin matrix protein1 (DMP1), and dentin sialophosphoprotein (DSPP) was inhibited by LOX-specific siRNA. In LOX enzyme activity,siRNA-induced knockdown of both LOX and LOXL inhibited the total amine oxidase activity in HDP cells, as in the case of mRNA expression. In conclusion, the essential role of H2O2 on odontoblastic differentiation suggests that its regulation by LOX may have pharmacologic importance in HDP cells.
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참고문헌 (26)
참고문헌 신청
Alliot-Licht B / 2005 / Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures / Cell Tissue Res 321 : 391 ~ 400
Beck GR Jr / 2000 / Phosphate is a specific signal for induction of osteopontin gene expression / Proc Natl Acad Sci U S A 97 : 8352 ~ 8357
Benetti AR / 2004 / In vitro penetration of bleaching agents into the pulp chamber / Int Endod J 37 : 120 ~ 124
Butler WT / 1995 / The nature and functional significance of dentin extracellular matrix proteins / Int J Dev Biol 39 : 169 ~ 179
Chang SW / 2009 / Heat stress activates interleukin-8 and the antioxidant system via Nrf2 pathways in human dental pulp cells / J Endod 35 : 1222 ~ 1228
Beck GR Jr / 2000 / Phosphate is a specific signal for induction of osteopontin gene expression / Proc Natl Acad Sci U S A 97 : 8352 ~ 8357
Benetti AR / 2004 / In vitro penetration of bleaching agents into the pulp chamber / Int Endod J 37 : 120 ~ 124
Butler WT / 1995 / The nature and functional significance of dentin extracellular matrix proteins / Int J Dev Biol 39 : 169 ~ 179
Chang SW / 2009 / Heat stress activates interleukin-8 and the antioxidant system via Nrf2 pathways in human dental pulp cells / J Endod 35 : 1222 ~ 1228
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