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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.6
- 수록면
- 368 - 376 (9page)
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초록· 키워드
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Background: In the current phytochemical research on ginseng, the differentiation and structural identification of ginsenosides isomers remain challenging. In this paper, a two-dimensional mass spectrometry (2D-MS) method was developed and combined with statistical analysis for the direct differentiation, identification, and relative quantification of protopanaxadiol (PPD)-type ginsenoside isomers.
Methods: Collision-induced dissociation was performed at successive collision energy values to produce distinct profiles of the intensity fraction (IF) and ratio of intensity (RI) of the fragment ions. To amplify the differences in tandem mass spectra between isomers, IF and RI were plotted against collision energy. The resulting data distributions were then used to obtain the parameters of the fitted curves, which were used to evaluate the statistical significance of the differences between these distributions via the unpaired t test.
Results: A triplet and two pairs of PPD-type ginsenoside isomers were differentiated and identified by their distinct IF and RI distributions. In addition, the fragmentation preference of PPD-type ginsenosides was determined on the basis of the activation energy. The developed 2D-MS method was also extended to quantitatively determine the molar composition of ginsenoside isomers in mixtures of biotransformation products.
Conclusion: In comparison with conventional mass spectrometry methods, 2D-MS provides more direct insights into the subtle structural differences between isomers and can be used as an alternative approach for the differentiation of isomeric ginsenosides and natural products.
Methods: Collision-induced dissociation was performed at successive collision energy values to produce distinct profiles of the intensity fraction (IF) and ratio of intensity (RI) of the fragment ions. To amplify the differences in tandem mass spectra between isomers, IF and RI were plotted against collision energy. The resulting data distributions were then used to obtain the parameters of the fitted curves, which were used to evaluate the statistical significance of the differences between these distributions via the unpaired t test.
Results: A triplet and two pairs of PPD-type ginsenoside isomers were differentiated and identified by their distinct IF and RI distributions. In addition, the fragmentation preference of PPD-type ginsenosides was determined on the basis of the activation energy. The developed 2D-MS method was also extended to quantitatively determine the molar composition of ginsenoside isomers in mixtures of biotransformation products.
Conclusion: In comparison with conventional mass spectrometry methods, 2D-MS provides more direct insights into the subtle structural differences between isomers and can be used as an alternative approach for the differentiation of isomeric ginsenosides and natural products.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusions
References
참고문헌 (41)
참고문헌 신청
State Pharmacopoeia Committee / 2010 / Pharmacopoeia of the People’s Republic of China / China Medical Science and Technology Press
Liu ZQ / 2012 / Chemical insights into ginseng as a resource for natural antioxidants / Chem Rev 112:3329~3355
Ganesan P / 2015 / Recent trends of nano bioactive compounds from ginseng for its possible preventive role in chronic disease models / RSC Adv 5:98634~98642
Wong AST / 2015 / Recent advances in ginseng as cancer therapeutics : a functional and mechanistic overview / Nat Prod Rep 32:256~272
Li KK / 2015 / A review on the medicinal potential of Panax ginseng saponins in diabetes mellitus / RSC Adv 5:47353~47366
Liu ZQ / 2012 / Chemical insights into ginseng as a resource for natural antioxidants / Chem Rev 112:3329~3355
Ganesan P / 2015 / Recent trends of nano bioactive compounds from ginseng for its possible preventive role in chronic disease models / RSC Adv 5:98634~98642
Wong AST / 2015 / Recent advances in ginseng as cancer therapeutics : a functional and mechanistic overview / Nat Prod Rep 32:256~272
Li KK / 2015 / A review on the medicinal potential of Panax ginseng saponins in diabetes mellitus / RSC Adv 5:47353~47366
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