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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.4
- 수록면
- 261 - 271 (11page)
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초록· 키워드
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Background: Protopanaxatriol (PPT) is an aglycone of ginsenosides, which has high medicinal values. Production of PPT from natural ginseng plants requires artificial deglycosylation procedures of ginsenosides via enzymatic or physicochemical treatments. Metabolic engineering could be an efficient technology for production of ginsenoside sapogenin. For PPT biosynthesis in Panax ginseng, damarenediol-II synthase (PgDDS) and two cytochrome P450 enzymes (CYP716A47 and CYP716A53v2) are essentially required.
Methods: Transgenic tobacco co-overexpressing P. ginseng PgDDS, CYP716A47, and CYP716A53v2 was constructed via Agrobacterium-mediated transformation.
Results: Expression of the three introduced genes in transgenic tobacco lines was confirmed by Reverse transcription-polymerase chain reaction (RT-PCR). Analysis of liquid chromatography showed three new peaks, dammarenediol-II (DD), protopanaxadiol (PPD), and PPT, in leaves of transgenic tobacco. Transgenic tobacco (line 6) contained 2.8 mg/g dry weight (DW), 7.3 mg/g DW, and 11.6 mg/g DW of PPT, PPD, and DD in leaves, respectively. Production of PPT was achieved via cell suspension culture and was highly affected by auxin treatment. The content of PPT in cell suspension was increased 37.25-fold compared with that of leaves of the transgenic tobacco. Transgenic tobacco was not able to set seeds because of microspore degeneration in anthers. Transmission electron microscopy analysis revealed that cells of phloem tissue situated in the center of the anther showed an abnormally condensed nuclei and degenerated mitochondria.
Conclusion: We successfully achieved the production of PPT in transgenic tobacco. The possible factors deriving male sterility in transgenic tobacco are discussed.
Methods: Transgenic tobacco co-overexpressing P. ginseng PgDDS, CYP716A47, and CYP716A53v2 was constructed via Agrobacterium-mediated transformation.
Results: Expression of the three introduced genes in transgenic tobacco lines was confirmed by Reverse transcription-polymerase chain reaction (RT-PCR). Analysis of liquid chromatography showed three new peaks, dammarenediol-II (DD), protopanaxadiol (PPD), and PPT, in leaves of transgenic tobacco. Transgenic tobacco (line 6) contained 2.8 mg/g dry weight (DW), 7.3 mg/g DW, and 11.6 mg/g DW of PPT, PPD, and DD in leaves, respectively. Production of PPT was achieved via cell suspension culture and was highly affected by auxin treatment. The content of PPT in cell suspension was increased 37.25-fold compared with that of leaves of the transgenic tobacco. Transgenic tobacco was not able to set seeds because of microspore degeneration in anthers. Transmission electron microscopy analysis revealed that cells of phloem tissue situated in the center of the anther showed an abnormally condensed nuclei and degenerated mitochondria.
Conclusion: We successfully achieved the production of PPT in transgenic tobacco. The possible factors deriving male sterility in transgenic tobacco are discussed.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (51)
참고문헌 신청
Christensen LP / 2009 / Ginsenosides chemistry, biosynthesis, analysis, and potential health effects / Adv Food Nutr Res 55:1~99
Xiang YZ / 2008 / A comparison of the ancient use of ginseng in traditional Chinese medicine with modern pharmacological experiments and clinical trials / Phytother Res 22:851~858
Leung KW / 2010 / Pharmacology of ginsenosides : a literature review / Chin Med 5:20
Nag SA / 2012 / Ginsenosides as anticancer agents : in vitro and in vivo activities, structure-activity relationships, and molecular mechanisms of action / Front Pharmacol 3:25
Shibata S / 2001 / Chemistry and cancer preventing activities of ginseng saponins and some related triterpenoid compounds / J Korean Med Sci 16:S28~S37
Xiang YZ / 2008 / A comparison of the ancient use of ginseng in traditional Chinese medicine with modern pharmacological experiments and clinical trials / Phytother Res 22:851~858
Leung KW / 2010 / Pharmacology of ginsenosides : a literature review / Chin Med 5:20
Nag SA / 2012 / Ginsenosides as anticancer agents : in vitro and in vivo activities, structure-activity relationships, and molecular mechanisms of action / Front Pharmacol 3:25
Shibata S / 2001 / Chemistry and cancer preventing activities of ginseng saponins and some related triterpenoid compounds / J Korean Med Sci 16:S28~S37
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