지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.4
- 수록면
- 308 - 314 (7page)
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초록· 키워드
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Two hundred and twenty three yeast strains were randomly isolated from Korean traditional nuruk, Among them, six urease producing yeast strains (designated JJA, JJB, JJ22, SHA, SHC and SH10) were selected on the Christensen urea agar plates. They showed the same pattern in the PCR-RFLP analysis of the ITS I-5.8S-ITS II region digested with HaeIII and HinF1 restriction endonucleases. Its DNA sequences showed 100%(strains SHA, SHC and SH10) and 99.8% (strains JJA, JJB and JJ22) identity with those of Issatchenkia orientalis type strain ATCC 24210. Phylogenetic analysis resulted in that all the strains were closely related to I. orientalis. Two representative strains, JJ22 and SH10, showing the highest urease activities were selected for further characterization. Their morphological, physiological and biochemical characteristics were also the same as I. orientalis. Therefore, both the two strains were identified as I. orientalis. They could grow at a wide range of temperature between 20-40℃ as well as pH between 2.0 and 10.0. However, a higher level urease activity were obtained at acidic pH than that at alkalic pH. The maximal level of rease activity was obtained at 30℃ (strain SH10) or 35℃ (strain JJ22) and in a liquid medium adjusted to the initial pH 5.0.
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참고문헌 (36)
참고문헌 신청
1. [학술지(정기간행물)] - Krajewska B / 2009 / Ureases I. Functional, catalytic and kinetic properties: A review / J Mol Cat B: Enzymatic 59 : 9 ~ 21
2. [학술지(정기간행물)] - Krajewska B / 2009 / Ureases. II. Properties and their customizing by enzyme immobilizations: A review / J Mol Cat B: Enzymatic 59 : 22 ~ 40
3. [학술지(정기간행물)] - Balasubramanian A / 2010 / Crystal structure of the first plant urease from Jack bean: 83 years of journey from its first crystal to molecular structure / J Mol Biol 400 : 274 ~ 283
4. [학술지(정기간행물)] - Miyagawa K / 1999 / Purification, characterization, and application of an acid urease from Arthrobacter mobilis / J Biotechnol 68 : 227 ~ 236
5. [학술지(정기간행물)] - Karmali K / 2004 / The use of Fourier transform infrared spectroscopy to assay for urease from Pseudomonas aeruginosa and Canavalia ensiformis / Anal Biochem 331 : 115 ~ 121
2. [학술지(정기간행물)] - Krajewska B / 2009 / Ureases. II. Properties and their customizing by enzyme immobilizations: A review / J Mol Cat B: Enzymatic 59 : 22 ~ 40
3. [학술지(정기간행물)] - Balasubramanian A / 2010 / Crystal structure of the first plant urease from Jack bean: 83 years of journey from its first crystal to molecular structure / J Mol Biol 400 : 274 ~ 283
4. [학술지(정기간행물)] - Miyagawa K / 1999 / Purification, characterization, and application of an acid urease from Arthrobacter mobilis / J Biotechnol 68 : 227 ~ 236
5. [학술지(정기간행물)] - Karmali K / 2004 / The use of Fourier transform infrared spectroscopy to assay for urease from Pseudomonas aeruginosa and Canavalia ensiformis / Anal Biochem 331 : 115 ~ 121
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UCI(KEPA) : I410-ECN-0101-2018-059-003065818