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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.8
- 수록면
- 604 - 611 (8page)
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Several wild yeasts were isolated from Korean grape varieties before and during spontaneous fermentation. Among them, four strains were isolated based on the alcohol content and flavor production in wine after fermentation of apple juice. In this study, the four yeast strains were identified and characterized. PCR-restriction fragment length polymorphism analysis of ITS I-5.8S-ITS II region with restriction endonuclease Hae Ⅲ and Hinf Ⅰ resulted in that all the strains showed a typical pattern of Saccharomyces cerevisiae. Pulse field gel electrophoresis showed three different chromosome patterns with a same band between strains SS89 and SS812. When ITS I-5.8S-ITS II sequences of the four strains were compared with one another, they were similar to those of Saccharomyces cerevisiae CBS 4054 type strain. Identity of the sequences was higher than 97% with those of the type strain. Phylogenetic analysis showed based on the sequences showed they were genetically closed to the type strain. The four identified strains were tested in a medium containing 200 ppm potassium metabisulfite, and the MM10 and WW108 inhibition rates resulted at up to 24 h. The four strains were tested at an incubation temperature of 30℃. The 30% sugar concentration in the medium (w/v) showed the highest growth in 36 h, especially in the case of SS89, which was close to growth 40. The four strains were tested in an 8% ethanol medium (v/v). Alcohol tolerance was initially kept in the incubation process. The strains began to adapt, however, to the exceeded resistance. The four strains showed the lowest inhibition rate at 24 h.
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참고문헌 (24)
참고문헌 신청
1. [단행본] - Montville TJ / 2005 / Food microbiology: an introduction / ASM Press : 223 ~ 239
2. [단행본] - Kurtzman CP / 1998 / The Yeasts, A Taxonomic Study(fourth edition) / Elsevier : 358 ~ 371
3. [학술지(정기간행물)] - Attfield PV / 1997 / Stress tolerance. The key to effective strains of industrial baker's yeast / Nat Biotechnol 15 : 1351 ~ 1357
4. [학술지(정기간행물)] - Pigeau G / 2005 / Upregulation of ALD3 and GPD1 in Saccharomyces cerevisiae during Icewine fermentation / J Appl Microbiol 99 : 112 ~ 125
5. [학술지(정기간행물)] - Erasmus DJ / 2003 / Genome-wide expression analyses: metabolic adaptation of Saccharomyces cerevisiae to high sugar stress / FEMS Yeast Res 2 : 375 ~ 399
2. [단행본] - Kurtzman CP / 1998 / The Yeasts, A Taxonomic Study(fourth edition) / Elsevier : 358 ~ 371
3. [학술지(정기간행물)] - Attfield PV / 1997 / Stress tolerance. The key to effective strains of industrial baker's yeast / Nat Biotechnol 15 : 1351 ~ 1357
4. [학술지(정기간행물)] - Pigeau G / 2005 / Upregulation of ALD3 and GPD1 in Saccharomyces cerevisiae during Icewine fermentation / J Appl Microbiol 99 : 112 ~ 125
5. [학술지(정기간행물)] - Erasmus DJ / 2003 / Genome-wide expression analyses: metabolic adaptation of Saccharomyces cerevisiae to high sugar stress / FEMS Yeast Res 2 : 375 ~ 399
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UCI(KEPA) : I410-ECN-0101-2018-059-003064846