지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 42 - 49 (8page)
이용수
초록· 키워드
오류제보하기
Background: Ginsenoside F1 has been described to possess skin-whitening effects on humans.We aimed to synthesize a new ginsenoside derivative from F1 and investigate its cytotoxicity and melanogenesis inhibitory activity in B16BL6 cells using recombinant glycosyltransferase enzyme. Glycosylation has the advantage of synthesizing rare chemical compounds from common compounds with great ease.
Methods: UDP-glycosyltransferase (BSGT1) gene from Bacillus subtilis was selected for cloning. The recombinant glycosyltransferase enzyme was purified, characterized, and utilized to enzymatically transform F1 into its derivative. The new product was characterized by NMR techniques and evaluated by MTT, melanin count, and tyrosinase inhibition assay.
Results: The new derivative was identified as (20S)-3β,6α,12β,20-tetrahydroxydammar-24-ene-20-O-β-D-glucopyranosyl-3-O-β-D-glucopyranoside (ginsenoside Ia), which possesses an additional glucose linked into the C-3 position of substrate F1. Ia had been previously reported; however, no in vitro biological activity was further examined. This study focused on the mass production of arduous ginsenoside Ia from accessible F1 and its inhibitory effect of melanogenesis in B16BL6 cells. Ia showed greater inhibition of melanin and tyrosinase at 100 μmol/L than F1 and arbutin. These results suggested that Ia decreased cellular melanin synthesis in B16BL6 cells through downregulation of tyrosinase activity.
Conclusion: To our knowledge, this is the first study to report on the mass production of rare ginsenoside Ia from F1 using recombinant UDP-glycosyltransferase isolated from B. subtillis and its superior melanogenesis inhibitory activity in B16BL6 cells as compared to its precursor. In brief, ginsenoside Ia can be applied for further study in cosmetics.
Methods: UDP-glycosyltransferase (BSGT1) gene from Bacillus subtilis was selected for cloning. The recombinant glycosyltransferase enzyme was purified, characterized, and utilized to enzymatically transform F1 into its derivative. The new product was characterized by NMR techniques and evaluated by MTT, melanin count, and tyrosinase inhibition assay.
Results: The new derivative was identified as (20S)-3β,6α,12β,20-tetrahydroxydammar-24-ene-20-O-β-D-glucopyranosyl-3-O-β-D-glucopyranoside (ginsenoside Ia), which possesses an additional glucose linked into the C-3 position of substrate F1. Ia had been previously reported; however, no in vitro biological activity was further examined. This study focused on the mass production of arduous ginsenoside Ia from accessible F1 and its inhibitory effect of melanogenesis in B16BL6 cells. Ia showed greater inhibition of melanin and tyrosinase at 100 μmol/L than F1 and arbutin. These results suggested that Ia decreased cellular melanin synthesis in B16BL6 cells through downregulation of tyrosinase activity.
Conclusion: To our knowledge, this is the first study to report on the mass production of rare ginsenoside Ia from F1 using recombinant UDP-glycosyltransferase isolated from B. subtillis and its superior melanogenesis inhibitory activity in B16BL6 cells as compared to its precursor. In brief, ginsenoside Ia can be applied for further study in cosmetics.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusion
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A systematic review of translation and experimental studies on internal anal sphincter for fecal incontinence
Annals of Coloproctology
2022 .06
-
The Gender-Sensitive Social Risk Factors for Internet Addiction in College Undergraduate Students
PSYCHIATRY INVESTIGATION
2021 .01
-
Internet Addiction and Its Associations with Clinical and Psychosocial Factors in Medical Students
PSYCHIATRY INVESTIGATION
2021 .01
-
Prevalence of Intracranial Aneurysm in Patients with Aortopathy: A Systematic Review with Meta-Analyses
대한뇌졸중학회지
2020 .01
-
Intracorporeal versus extracorporeal anastomosis in laparoscopic right hemicolectomy: a retrospective cohort study of anastomotic complications
Annals of Coloproctology
2023 .04
-
The Current Situation of Internet Addiction and Its Impact on Sleep Quality and Self-Injury Behavior in Chinese Medical Students
PSYCHIATRY INVESTIGATION
2020 .01
-
Polymorphisms of Inflammatory Cytokine Genes and Risk for Intracranial Aneurysm: A Systematic Review and Meta-Analysis
Yonsei Medical Journal
2020 .01
-
인터넷 중독에서 생물학적 지표로써 사건유발전위 적용
중독정신의학
2019 .01
-
A Novel Association between Lysyl Oxidase Gene Polymorphism and Intracranial Aneurysm in Koreans
Yonsei Medical Journal
2017 .01
-
Associations among Genetic Variants and Intracranial Aneurysm in a Chinese Population
Yonsei Medical Journal
2019 .01
-
저령 추출물의 멜라닌 생성억제 작용
Korean Journal of Acupuncture
2020 .01
-
Control of Motility in the Internal Anal Sphincter
Journal of Neurogastroenterology and Motility (JNM)
2019 .01
-
Recent advances in intra-articular drug delivery systems to extend drug retention in joint
Journal of Pharmaceutical Investigation
2019 .01
-
성인기에 진단된 당원병 제 Ia형의 다양한 임상 양상
대한유전성대사질환학회지
2015 .01
-
Biosynthesis of rare 20(R)-protopanaxadiol/protopanaxatriol type ginsenosides through Escherichia coli engineered with uridine diphosphate glycosyltransferase genes
Journal of Ginseng Research
2019 .01
-
Prediction of Ambulatory Status After Hip Fracture Surgery in Patients Over 60 Years Old
Annals of Rehabilitation Medicine
2016 .01
-
How Cerebral Vessel Tortuosity Affects Development and Recurrence of Aneurysm: Outer Curvature versus Bifurcation Type
대한뇌졸중학회지
2021 .01
-
Comparing extracorporeal, semi-extracorporeal, and intracorporeal anastomosis in laparoscopic right hemicolectomy: introducing a bridging technique for colorectal surgeons
Annals of Surgical Treatment and Research
2024 .07
-
Bacillus spp. or Bacillus spp.-Derived Membrane Vesicles Induce the Intrinsic Pathways of Apoptosis of Human Colon Cancer Cell Lines
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2016 .06
-
간부전 환자는 신부전 환자보다 IgG 동종응집소 역가가 높다
대한수혈학회지
2021 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2018-524-001744532