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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.10
- 수록면
- 351 - 358 (8page)
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초록· 키워드
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Background: This study was designed to investigate whether ginsenoside Rb1 (Rb1) and compound K (CK) ameliorated insulin resistance by suppressing endoplasmic reticulum (ER) stress-induced inflammation in adipose tissue.
Methods: To induce ER stress, epididymal adipose tissue from mice or differentiated 3T3 adipocytes were exposed to high glucose. The effects of Rb1 and CK on reactive oxygen species production, ER stress, TXNIP/NLRP3 inflammasome activation, inflammation, insulin signaling activation, and glucose uptake were detected by western blot, emzyme-linked immunosorbent assay, or fluorometry.
Results: Rb1 and CK suppressed ER stress by dephosphorylation of IRE1α and PERK, thereby reducing TXNIP-associated NLRP3 inflammasome activation in adipose tissue. As a result, Rb1 and CK inhibited IL-1β maturation and downstream inflammatory factor IL-6 secretion. Inflammatory molecules induced insulin resistance by upregulating phosphorylation of insulin receptor substrate-1 at serine residues and impairing insulin PI3K/Akt signaling, leading to decreased glucose uptake by adipocytes. Rb1 and CK reversed these changes by inhibiting ER stress-induced inflammation and ameliorating insulin resistance, thereby improving the insulin IRS-1/PI3K/Akt-signaling pathway in adipose tissue.
Conclusion: Rb1 and CK inhibited inflammation and improved insulin signaling in adipose tissue by suppressing ER stress-associated NLRP3 inflammation activation. These findings offered novel insight into the mechanism by which Rb1 and CK ameliorate insulin resistance in adipose tissue.
Methods: To induce ER stress, epididymal adipose tissue from mice or differentiated 3T3 adipocytes were exposed to high glucose. The effects of Rb1 and CK on reactive oxygen species production, ER stress, TXNIP/NLRP3 inflammasome activation, inflammation, insulin signaling activation, and glucose uptake were detected by western blot, emzyme-linked immunosorbent assay, or fluorometry.
Results: Rb1 and CK suppressed ER stress by dephosphorylation of IRE1α and PERK, thereby reducing TXNIP-associated NLRP3 inflammasome activation in adipose tissue. As a result, Rb1 and CK inhibited IL-1β maturation and downstream inflammatory factor IL-6 secretion. Inflammatory molecules induced insulin resistance by upregulating phosphorylation of insulin receptor substrate-1 at serine residues and impairing insulin PI3K/Akt signaling, leading to decreased glucose uptake by adipocytes. Rb1 and CK reversed these changes by inhibiting ER stress-induced inflammation and ameliorating insulin resistance, thereby improving the insulin IRS-1/PI3K/Akt-signaling pathway in adipose tissue.
Conclusion: Rb1 and CK inhibited inflammation and improved insulin signaling in adipose tissue by suppressing ER stress-associated NLRP3 inflammation activation. These findings offered novel insight into the mechanism by which Rb1 and CK ameliorate insulin resistance in adipose tissue.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (28)
참고문헌 신청
[학술지(정기간행물)] - Hager SR / 1991 / Insulin resistance in normal rats infused with glucose for 72 h / Am J Physiol 260 : E353 ~ E362
[학술지(정기간행물)] - Rossetti L / 1995 / Glucose toxicity: the implications of hyperglycemia in the pathophysiology of diabetes mellitus / Clin Invest Med 18 : 255 ~ 260
[학술지(정기간행물)] - Haber CA / 2003 / N-acetylcysteine and taurine prevent hyperglycemia-induced insulin resistance in vivo: possible role of oxidative stress / Am J Physiol Endocrinol Metab 285 : E744 ~ E753
[학술지(정기간행물)] - Dedoussis GV / 2007 / Genes, diet and type 2 diabetes mellitus: a review / Rev Diabet Stud 4 : 13 ~ 24
[학술지(정기간행물)] - Eizirik DL / 2008 / The role for endoplasmic reticulum stress in diabetes mellitus. / Endocr Rev 29 : 42 ~ 61
[학술지(정기간행물)] - Rossetti L / 1995 / Glucose toxicity: the implications of hyperglycemia in the pathophysiology of diabetes mellitus / Clin Invest Med 18 : 255 ~ 260
[학술지(정기간행물)] - Haber CA / 2003 / N-acetylcysteine and taurine prevent hyperglycemia-induced insulin resistance in vivo: possible role of oxidative stress / Am J Physiol Endocrinol Metab 285 : E744 ~ E753
[학술지(정기간행물)] - Dedoussis GV / 2007 / Genes, diet and type 2 diabetes mellitus: a review / Rev Diabet Stud 4 : 13 ~ 24
[학술지(정기간행물)] - Eizirik DL / 2008 / The role for endoplasmic reticulum stress in diabetes mellitus. / Endocr Rev 29 : 42 ~ 61
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UCI(KEPA) : I410-ECN-0101-2018-524-001598476