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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.6
- 수록면
- 103 - 107 (5page)
- DOI
- 10.7841/ksbbj.2017.32.2.103
이용수
초록· 키워드
오류제보하기
The effect of the trehalose incorporation on the biological membranes was investigated with respect to the phase of the membranes using the fluorescence intensity change. Spherical phospholipid bilayers, vesicles, were prepared only with the variation in the phase of each layer via a double emulsion technique. In the aqueous inside of the vesicles, 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt(ANTS) was encapsulated. As a quencher, p-Xylene-bis(N-pyridinium bromide)(DPX) was included in the buffer where the vesicles were dispersed. The fluorescence scale was calibrated with the fluorescence of ANTS vesicles in p-Xylene-bis(N-pyridinium bromide)(DPX)-included-buffer taken as 100% fluorescence and the mixture of ANTS and DPX in the buffer as 0% fluorescence. Trehalose injection into the vesicle solution led the distortion of the membrane. It was found that the distortion was related to the phase of each layer the vesicle up on the ratio of trehalose to lipid. In the identical measurements at glucose, the behavior of the distortion was completely different from that of trehalose. These results seem to depend on the stability of the vesicles, due to the osmotic and volumetric effects on the headgroup packing disruption.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (31)
참고문헌 신청
Crowe, J. H. and L. M. Crowe (1992) Water and life, pp. 5-7. Springer, Berlin, Germany.
Crowe, J. H., L. M. Crowe, and S. A. Jackson (1983) Preservation of structural and functional activity in lyophilized sarcoplasmic reticulum. Arch. Biochem. Biophys. 220: 477-484.
Crowe, L. M., J. H. Crowe, A. Rudolph, C. Womersley, and L. Appel (1985) Preservation of freeze- dries liposomes by trehalose. Arch. Biochem. Biophys. 242: 240-247.
Leslie, S. B., S. A. Teter, L. M. Crowe, and J. H. Crowe (1994) Trehalose lowers membrane phase transitions in dry yeast cells. Biochim. Biophys. Acta 1192: 7-13.
Carpenter, J. F., L. M. Crowe, and J. H. Crowe (1987) Stabilization of dry phospholipid bilayers and proteins by sugars. Biochim. Biophys. Acta 923: 109-115.
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UCI(KEPA) : I410-ECN-0101-2018-470-000978006