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논문 기본 정보

자료유형
학술저널
저자정보
Thi Phuong Duyen Vu (Chungnam National University) Kyung Tae Kim (Chungnam National University) Yen Pham (University of Science and Technology of Hanoi) Haiying Bao (Jilin Agricultural University) Jong Seong Kang (Chungnam National University)
저널정보
한국분석과학회 분석과학 분석과학 제30권 제2호
발행연도
2017.4
수록면
82 - 88 (7page)

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초록· 키워드

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Adenophorae Radix (AR) is a frequently used medicinal herb; because of its popularity, products containing similar herbal products are often sold as substitutes, especially if their morphology is similar. However, any analytical method to identify AR based on quantitative analysis is not registered in Korea, Japan and China Pharmacopoeias. This study developed a simple HPLC method to discriminate between authentic AR and substitutes. Linoleic acid was used as a marker compound of AR. Our optimized HPLC-UV conditions included a mobile phase of 90 % acetonitrile under isocratic condition, and a flow rate of 1.0 mL/min at room temperature. Detection wavelength was set at 205 nm. Linoleic acid was detected at 13.5 minutes for a total analysis time of 20 minutes. The standard herb of AR contained 0.025 % of linoleic acid, while four authentic AR samples and eight substitutes contained 0.040~0.071 % and 0.004~0.014 %, respectively. Comparison of the linoleic acid concentrations of the sample types to reference AR showed that among 12 samples, only the four samples were authentic. Thus, our HPLC-UV method, along with our suggested content criterion for linoleic acid concentration, can be used for the quick and accurate determination whether the herbal products are authentic AR or substitute.

목차

Abstract
1. Introduction
2. Experimental
3. Results and Discussion
4. Conclusions
References

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