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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.43 No.4
- 발행연도
- 2016.12
- 수록면
- 575 - 580 (6page)
이용수
초록· 키워드
오류제보하기
This study was performed to determine whether supplementation of tauroursodeoxycholic acid (TUDCA), an endoplasmic reticulum (ER) stress inhibitor, during vitrified cryopreservation enhances the development of frozen mouse embryos. Mouse 8-cell stage embryos were collected and exposed to a cryoprotectant solution containing TUDCA or TM (tunicamycin, an ER stress inhibitor) at room temperature and stored in liquid nitrogen following vitrification. The final concentration of TUDCA or TM was 50 μM. The survival and development rates of mouse 8-cell stage embryos exposed to TUDCA- or TM-containing solutions at room temperature or stored in liquid nitrogen following vitrification were measured. There were no significant differences in survival rate and blastocyst formation rate among control, TUDCA, and TM groups after embryos were exposed to vitrification solutions at RT. When mouse 8-cell stage embryos were treated with TUDCA or TM and then stored in liquid nitrogen, the survival rates of control and TUDCA groups were significantly higher than for the TM group. Blastocyst formation rate of the TUDCA group following in vitro culture was significantly higher than that in control or TM groups. The TM group showed a lower (p < 0.05) blastocyst formation rate than the other two groups. Our results indicate that TUDCA supplementation during cryopreservation of mouse embryos could enhance their development capacity.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
References
참고문헌 (15)
참고문헌 신청
Gibbons A / 2011 / A simple vitrification technique for sheep and goat embryo cryopreservation / Small Ruminant Research 95:61~64
Groenendyk J / 2005 / Endoplasmic reticulum quality control and apoptosis / Acta Biochimca Polonica 52:381~395
Gualtieri R / 2009 / Slow cooling of human oocytes : Ultrastructural injuries and apoptotic status / Fertility and Sterility 91:1023~1034
Kim JS / 2012 / Tauroursodeoxycholic acid enhances the pre-implantation embryo development by reducing apoptosis in pigs / Reproduction in Domestic Animals 47:791~798
Latham KE / 2015 / Endoplasmic reticulum stress signaling in mammalian oocytes and embryos : Life in balance / International Review of Cell and Molecular Biology 316:227~265
Groenendyk J / 2005 / Endoplasmic reticulum quality control and apoptosis / Acta Biochimca Polonica 52:381~395
Gualtieri R / 2009 / Slow cooling of human oocytes : Ultrastructural injuries and apoptotic status / Fertility and Sterility 91:1023~1034
Kim JS / 2012 / Tauroursodeoxycholic acid enhances the pre-implantation embryo development by reducing apoptosis in pigs / Reproduction in Domestic Animals 47:791~798
Latham KE / 2015 / Endoplasmic reticulum stress signaling in mammalian oocytes and embryos : Life in balance / International Review of Cell and Molecular Biology 316:227~265
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UCI(KEPA) : I410-ECN-0101-2017-520-002299129